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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (10): 43-46.

所属专题： 生物技术； 园艺

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

PCR及核酸探针检测耐甲氧西林葡萄球菌mecA基因的研究

杜沂平,邹玲,刘文华,任慧英   

  • 收稿日期:2008-07-21 修回日期:2008-08-28 出版日期:2008-10-05 发布日期:2008-10-05

Detection of mecA Gene of Methicillin-resistant Staphylococcus by PCR and Nucleic Acid Probe

Yi-ping Du,Ling Zou,WENHUA LIU   

  • Received:2008-07-21 Revised:2008-08-28 Online:2008-10-05 Published:2008-10-05

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摘要： 为了解耐甲氧西林葡萄球菌在畜禽间的存在情况，本试验建立针对mecA基因的PCR方法及地高辛标记核酸探针技术，分别用两种方法对75株葡萄球菌分离株进行检测。结果，经PCR方法检出42株耐甲氧西林葡萄球菌，占分离株的56.0%；经核酸探针方法检出47株耐甲氧西林葡萄球菌，占分离株的62.7%，两种方法的总符合率为93.3%。通过本研究证实，在畜禽的体表及体内能够分离到耐甲氧西林葡萄球菌，且有较高的分离率，应该引起兽医工作者的足够重视。

Abstract: In order to investigate the situation of methicillin-resistant Staphylococcus(MRS) in livestock and poultry, the method of PCR and dig-labeled nucleic acid probe to mecA gene fragment were established to detect 75 Staphylococcus isolates. Forty-two strains of MRS were screened out by PCR, accounting for 56.0%. Forty-seven strains of MRS were screened out by nucleic acid probe method, accounting for 62.7%. The coincidence rate of acid probe method with PCR method was 93.3%. It was verified that MRS could be isolated from livestock and poultry in vivo or in vitro, with higher isolation rate. Veterinary researchers should pay more attention to Methicillin-resistant Staphylococcus.