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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (2): 55-58.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

白桦愈伤组织中三萜物质提取条件的优化研究

范桂枝,詹亚光,李康,翟俏丽   

  • 收稿日期:2008-10-13 修回日期:2008-10-22 出版日期:2009-01-20 发布日期:2009-01-20

Preliminary optimization the extration conditions of triterpene in callus of Betula platyphylla Suk.

  • Received:2008-10-13 Revised:2008-10-22 Online:2009-01-20 Published:2009-01-20

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摘要： （研究目的）为了提高白桦愈伤组织中的三萜产量，（方法）本实验以齐墩果酸为标品，以5%香草醛-冰乙酸溶液、高氯酸为显色系统，对白桦愈伤组织中三萜含量的测定进行了评价，（结果）发现该方法具有较高的重现性，并且显色液在5~50min内测定吸光值较稳定；对白桦三萜的提取温度和试剂优化发现，白桦愈伤组织样品在20℃，40℃，60℃，80℃等温度下进行超声提取，其三萜含量依次为40℃（1.67mg/g）＞ 60℃（1.28 mg/g）＞ 80℃（0.47 mg/g）＞ 20℃（0.08 mg/g）。在有机溶剂中，其含量依次为：苯（2.58 mg/g）＞ 乙醚（2.04 mg/g）＞ 乙醇（1.67 mg/g）＞ 氯仿（1.21 mg/g）＞ 甲醇（0.89 mg/g）＞ 丙酮（0.50 mg/g）。（结论）因此建议，白桦愈伤组织中三萜提取的最佳温度为40℃，提取试剂为95%乙醇。

关键词: 农业科技园, 农业科技园, 傅家边

Abstract: （OBJECTIVE）To improve the yield of triterpene in callus of birch, (METHOD）oleanolic acid as standard, 5% vanillin-acetic acid and perchloric acid as the developer, the determination method of triterpene was evaluated, the results showed that this method had a higher repeatability, and quite stable during 5~50min in color reaction; The triterpene in callus with ultrasonic was extracted under 20℃，40℃，60℃，80℃, the content of triterpene was 40℃（1.67mg/g）＞ 60℃（1.28 mg/g）＞ 80℃（0.47 mg/g）＞ 20℃（0.08 mg/g）. The triterpene in callus was extracted with benzene, acetone, ether, ethanol95%, methanol, chloroform, the content of triterpene was benzene（2.58 mg/g）＞ ether（2.04 mg/g）＞ ethanol（1.67 mg/g）＞ chloroform（1.21 mg/g）＞ methanol（0.89 mg/g）＞ acetone（0.50 mg/g）. （CONCLUSION） It is proposed that the best extration temperature and reagent was 40℃ and ethanol95%,respectively.