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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (5): 195-199.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

两种观赏水草的离体培养

王晓萍,吴丽爽,孙玉刚   

  • 收稿日期:2008-11-10 修回日期:2009-01-21 出版日期:2009-03-05 发布日期:2009-03-05

Regeneration of two types of Ornamental waterweeds

  • Received:2008-11-10 Revised:2009-01-21 Online:2009-03-05 Published:2009-03-05

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摘要： 本试验以两种观赏水草为供试材料，进行离体培养研究。试验结果表明：红波（Alternanthera bettzickiana）以叶片为外植体，愈伤组织诱导采用MS+6-BA0.5 mg/L (单位下同)+NAA0.2的培养基，芽诱导采用MS+6-BA2.0 +NAA0.05+AD10的培养基，茎尖芽诱导采用MS+6-BA2.0+NAA0.05培养基，继代培养采用MS+6-BA1.0 +NAA0.05的培养基，根诱导采用1/2MS+IBA0.5的培养基；金钱草（Lysima chiachristinae Hance）以茎尖为外植体，芽诱导采用MS+6-BA0.5+NAA0.02的培养基，继代培养用MS+6-BA0.5+NAA0.05的培养基，根诱导采用1/2MS+IBA0.5的培养基，上述培养基中均含有30 g/L蔗糖和6g/L琼脂，PH5.6，在培养温度25℃、光照强度1800Lx、光照时间12h/d的培养条件下，均能培育出完整植株。

关键词: “三农”, “三农”, 农民利益, 思路

Abstract: Two kinds of ornamental waterweeds were used as tested material in this study. The results showed that: the leaves of Alternanthera bettzickiana were used as explants to initiate callus on MS supplemented with 6-BA0.5 mg/L (units the same below) and NAA0.2; bud was induced on MS supplemented with 6-BA2.0, NAA0.05,AD10; shoot-tip bud was induced on MS supplemented with 6-BA2.0, NAA0.05; subcultured on MS supplemented with 6-BA1.0 ,NAA0.05; root was induced on 1/2MS supplemented with IBA0.5. Shoot tips of Lysima chiachristinae Hance were used as explants to initiate buds on MS supplemented with 6-BA0.5,NAA0.02; subcultured on MS supplemented with 6-BA0.5, NAA0.05; roots were induced on 1/2MS supplemented with IBA0.5. The medium contained 30g /L sucrose and 6 g /L agar. The cultures were maintained at 25℃ under a 12-h photoperiod with light intensity 1800 Lx, can produce complete plants.