欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (10): 81-83.

• 生物技术科学 • 上一篇    下一篇

芸芥（E.Sativa Mill.）柱头总RNA提取方法比较研究

白生文,孙万仓,范惠玲   

  • 收稿日期:2008-12-12 修回日期:2009-03-23 出版日期:2009-05-20 发布日期:2009-05-20

Comparison and Improvement of Different Extracting Total RNA Methods from Stigmas in E.Sativa Mill.

  • Received:2008-12-12 Revised:2009-03-23 Online:2009-05-20 Published:2009-05-20

PDF (PC)

713

摘要： 本文在常规异硫氰酸胍法的基础上,改进并确立了一种适合芸芥柱头总RNA的提取与纯化方法。结果表明，该方法所提取的芸芥柱头总RNA不但完整性好、纯度高, 消除了DNA、蛋白质、多糖和多酚类物质的污染,可用于cDNA合成、mRNA差异显示和Northern杂交等分子生物学研究，而且操作简单，经济，实验结果稳定，重复性好，还适合于较多材料RNA的提取。

关键词: 青菜, 青菜, TPS不育系, 杂交制种, 行比

Abstract: On the basis of guanidine thiocyanate method, an economical method of extracting and puring total RNA from Yunjie stigmas was established.The results showed that the total RNA were in good integrity and high purity, which demonstrated that RNA samples were free of contamination of protein,DNA,polysaccharide and polyphenol ,and were applicable to cDNA synthesis,DDRT-PCR,northern blotting and so on.This method was simple,economic, and of stable performance, good repeatability as well as suitable for extracting total RNA from many materials.