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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (7): 351-355.

所属专题： 生物技术； 园艺

• 植物保护 农药 • 上一篇    下一篇

瓜类尖孢镰刀菌致病物质β-D-葡萄糖苷酶活性与致病性的相关性分析

肖荣凤,蓝江林,车建美,刘 波,刘丹莹   

  • 收稿日期:2008-03-18 修回日期:2008-04-24 出版日期:2008-07-05 发布日期:2008-07-05

Study on the Correlation between the Activity of β-D-glucosidase and the Pathogenicity of Fusarium oxysporum Strains from Cucurbitaceous Crops

Xiao Rongfeng, Lan Jiangling, Che Jianmei, Liu Bo, Liu Danying   

  • Received:2008-03-18 Revised:2008-04-24 Online:2008-07-05 Published:2008-07-05

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摘要： 【研究目的】研究瓜类尖孢镰刀菌β-D-葡萄糖苷酶活性与致病性的关系，为菌株致病性强弱快速测定提供一种新的辅助方法；【方法】测定了19株来自不同瓜类寄主尖孢镰刀菌菌株的致病性及致病物质β-D-葡萄糖苷酶活性，构建菌株的发病率(y)与酶活(x)的曲线模型；【结果】菌株致病性测定结果表明，同一寄主及不寄主来源的菌株致病性有差异。供试菌株中有12个强致病菌株，发病率在80%以上；2个中等致病菌株，发病率分别为60%和70%；2个弱致病菌株发病率均为30%，以及3个非致病菌株。菌株β-D-葡萄糖苷酶活测定结果表明，同一寄主不同菌株的酶活存在明显差异，来自黄瓜、甜瓜和西瓜寄主菌株酶活变化范围依次为（21.57-54.89）、（16.28-41.83）和（14.98-32.43）U•ml-1；不同寄主（黄瓜、甜瓜和西瓜）菌株的β-D-葡萄糖苷酶活也存在差异，平均酶活为黄瓜（34.08 U•ml-1）甜瓜（27.86 U•ml-1）西瓜（20.95 U•ml-1）；【结论】菌株的β-D-葡萄糖苷酶活性与其致病性关系分析表明，当酶活性小于30 U•ml-1l时，菌株发病率与酶活呈正相关；当酶活大于30 U•ml-1，菌株的发病率大于90%。

关键词: 黄羽肉种鸡, 黄羽肉种鸡, 溴隐停, 产蛋性能, 血浆雌二醇含量

Abstract: 【OBJECTIVE】Studying the correlation of pathogenicity and β-D-glucosidase activityof strains in order to find the fast testing method of the pathogenicity of Fusarium oxysporum from cucurbitaceous crops.【METHOD】we tested the pathogenicity and β-D-glucosidase activity for nineteen strains isolated from cucumber, melon and watermelon, and form a correlation construct of pathogenicity(y) and β-D-glucosidase activity(x).【RESULTS】The results showed that significant differences of the pathogenicity and β-D-glucosidase activity were found among different strains from the same or the different host-plants. The tested strains were grouped into pathogenicity and nonpathogenicity, and the β-D-glucosidase activity of strains from cucumber, melon and watermelon were in the range of 21.57-54.89，16.28-41.83 and 14.98-32.43 U•ml-1, respectively. The average activity of the strains from cucumber, melon and watermelon were 34.08, 27.86, 20.95 U•ml-1.【CONCLUSION】There was a positive correlation between the β-D-glucosidase activity and pathogenicity of F. oxysporum isolated from cucurbitaceous crops while the activity was less than 30 U•ml-1; contrarily , disease incidence of strains were more than 90% while more than 30 U•ml-1.