相关序列扩增多态性(SRAP)一种新的分子标记技术

中国农学通报 ›› 2004, Vol. 20 ›› Issue (6): 11-11.

Sequence-related Amplified Polymorphism(SRAP)：A Novel Technique for Molecular Marker

Ren Yu, Wang Deyuan Zhang Yindong

Online:2004-12-05 Published:2004-12-05

摘要/Abstract

摘要： SRAP是一种新的DNA分子标记，具有简便、稳定、中等产率和容易得到选择条带序列的特点。SRAP是一种基于PCR技术的新的分子标记技术，其利用独特的引物设计对ORFs进行扩增，上游引物长17bp，对外显子进行特异扩增，下游引物长18bp，对内含子区域、启动子区域进行特异扩增，因个体不同以及物种的内含子、启动子与间隔长度不等而产生多态性。扩增产物用变性的聚丙烯酰胺凝胶分离，然后用放射自显影检测。本文在阐述SRAP的原理和流程后，详细论述了SRAP标记目前在植物遗传图谱构建、遗传多样性、基因定位、比较基因组学、杂种优势预测等方面的研究进展及应用前景。

Abstract: SRAP is a new technique for molecular marker .It is simplicity, reliability, moderate throughput ratio and facile sequencing of selected bands. This paper describes the SRAP principle, protocol. SRAP aimed for amplification of open reading frames(ORFs) by special primer set. The forward prime of 17 base amplified exons, the reverse primer of 18 base amplified introns and promoters. Since introns, promoters and spacers are variable among different individuals, this makes it feasible to generate polymorphism. The amplified DNA fragments are separated by denaturing acrylamide gels and detected by autoradiography. This technique has been used in genetics mapping, genetic diversity, gene tagging, comparative genomics and heterosis prediction. The prospect of the SRAP marker application in plant genetics and research were discussed.

任羽,,王得元,张银东. 相关序列扩增多态性(SRAP)一种新的分子标记技术[J]. 中国农学通报, 2004, 20(6): 11-11.

Ren Yu, Wang Deyuan Zhang Yindong. Sequence-related Amplified Polymorphism(SRAP)：A Novel Technique for Molecular Marker[J]. Chinese Agricultural Science Bulletin, 2004, 20(6): 11-11.