UV-B与植物DNA: 损伤与修复

摘要/Abstract

摘要： 臭氧衰减导致地表紫外辐射增强，而紫外线辐射对植物表皮细胞的DNA 具有损害作用，可导致环丁烷嘧啶二聚体和6-4 光产物的形成。植物DNA对UV-B辐射的响应具有一定的敏感性，这种敏感性与不同的UV-B辐射剂量和不同植物细胞的原生质体有关，表现出一定的剂量—效应关系。DNA损伤的修复在生物体内普遍存在。植物可以通过多种途径来修复紫外诱导的DNA损伤，主要包括光修复、碱基切除修复、核苷酸切除修复和重组修复等。DNA 的修复能力与紫外辐射的剂量在一定程度上呈正相关。本文概述了对紫外辐射引起的DNA损伤、植物DNA对UV-B辐射响应的敏感性、损伤的修复和未来的研究方向。

Abstract: Stratospheric ozone depletion enhanced solar UV-B radiation reaching the earth surface, resulting in damaging to DNA of plant epidermal cells, induced cyclobutane pyrimidine dimmers (CPD) and 6-4 photoproducts. The sensitivity of plant DNA to UV-B was related with different UV-B radiation intensity and different plant cytoplast, and the dose-effect relationship was showed. Generally, the organisms could repair DNA damage induced by UV-B radiation. Plant could repair DNA damage induced by UV-B through many methods, mainly included light-repair, base excision repair, nucleotide excision repair and recombination repair etc. The reparation of DNA damage was positively related with UV-B radiation intensity，generally. The contents were reviewed in this paper involved DNA damage induced by UV-B, The sensitivity of plant DNA to UV-B radiation, reparation of DNA damage and further research keys.

何永美,祖艳群,李元. UV-B与植物DNA: 损伤与修复[J]. 中国农学通报, 2009, 25(14): 42-46.

He Yongmei, Zu Yanqun, Li Yuan. UV-B and Plant DNA：Damage and Repair[J]. Chinese Agricultural Science Bulletin, 2009, 25(14): 42-46.

参考文献

[1] Rajeshwar PS, Donat PH. UV-induced DNA damage and repair: a review. Photochemistry Photobiology science, 2002, 1:225-236.
[2] Teranishi M. Ultraviolet-B sensitivities in Japanese lowland rice cultivars: cyclobutane pyrimidine dimer photolyase activity and gene mutation. Plant Cell Physiol, 2004, 45(12):1848-1856.
[3] Toyotaka I, Seisuke K, Tomoyuki F, et al. Review DNA Repair Mechanisms in UV-B Tolerant Plants .Jarq , 2006, 40(2):107-113.
[4] Kaiserli E, Jenkins GI. UV-B promotes rapid nuclear translocation of the Arabidopsis UV-B-speciﬁc signaling component UVR8 and activates its function in the nucleus, Plant Cell, 2007, 19:2662-2673.
[5] Hidemal J, Taguchil T, Onol T, et al, Increase in CPD photolyase activity functions effectively to prevent growth inhibition caused by UV-B radiation. Plant Journal, 2007, 50:70-79.
[6] 贺军民,王瑞斌,孟朝妮.绿豆幼苗UV-B 敏感性与环丁烷嘧啶二聚体累积的关系.中国农业科学,2006,39(7):1358-1364.
[7] 王艳,李韶山,徐宁,等.UV-B 诱导水稻叶片 DNA 形成 CPD 的研究.激光生物学报,2001,10(2):101-103.
[8] Hanns F, Dorothee S. Ultraviolet-B Radiation-Mediated Responses in Plants: Balancing Damage and Protection. Plant Physiology, 2003, 133:1420-1428.
[9] Gichner T, Patkova’ Z, Sza’kova’ J, et al. Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves, Mutat Research. 2004, 9:49-57.
[10] Schmitz HR, Weissenböck, G. Contribution of phenolic compounds to the UV-B screening capacity of developing barley primary leaves in relation to DNA damage and repair under elevated UV-B levels. Phytochemistry, 2003, 64(1):243-255.
[11] 强维亚,汤红官,侯宗东.增强UV-B辐射对大豆胚轴DNA损伤、修复和蛋白质含量的影响.生态学报,2004,24(4):852-857.
[12] 王静,蒋磊,王艳,等.紫外辐射诱导植物叶片DNA损伤敏感性差异.植物学通报,2007,24(2):189-193.
[13] Manetas Y. The importance of being hairy: The adverse effects of hair removal on stem photosynthesis of Verbascum speciosum are due to solar UV -B radiation. New Phytol., 2003, 3: 503-50.
[14] Ballare CL, Scopel AL, Stapleton AE. Solar ultraviolet radiation affects seedling emergence, DNA integrity, plant morphology, growth rate, and attractiveness to herbivore insects in Datura feros. Plant Physiol. 1996, 112: 161-170.
[15] 何丽莲,祖艳群,李元,等.小麦品种对增强UV-B辐射响应的RAPD分析. 农业环境科学学报, 2006, 25(1):73-76.
[16] Kimura S, Tahira Y, Ishibashi T. DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells, Nucleic Acids Research. 2004, 32:2760-2767.
[17] Narendra T, Parvaiz A, Brahma BP. Genotoxic stress in plants: Shedding light on DNA damage, repair and DNA repair helicases，Mutat. Res. 2008, 4:1016-1032.
[18] Sakamoto A, Lan VT, Hase Y, et al. Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants. Plant Cell, 2003, 15(9):2042-2057.
[19] Ries G, Hellea W, Puchta H. Elevated UV-B radiation reduces genome stability in plants，Nature, 2000, 406: 98-101.
[20] Crowley DJ, Boubriak I, Berquist BR, et al. The uvrA, uvrB and uvrC genes are required for repair of ultraviolet light induced DNA photoproducts in Halobacterium sp. NRC-1. Saline Systems, 2006, 2:11.
[21] Fan L, Arvai AS, Cooper PK, et al. Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair, Molecular Cell, 2006, 22:27-37.
[22] Van HA, Balajee AS, Van ZAA, et al. Nucleotide excision repair and its interplay with transcription. Toxicology, 2003, 193(1-2):79-90.
[23] Groisman R, Polanowska J, Kuraoka I, et al. The ubiquityin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell .2003:113(3):357-367.
[24] Winkler GS, Araujo SJ, Fiedler U, et al.TFIIH with inactive XPD helicase functions in transcription initiation but is defective in DNA repair. Journal of Photochemistry and Photobiology. 2000, 275:4258-4266.
[25] Coin F, Oksenych V, Egly JM. Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair. Molecular Cell. 2007, 26:245-256.
[26] Collins AR. The comet assay for DNA damage and repair: principles, applications and limitations. Molecular Biotechnology. 2004, 26:249-261.
[27] Hder DP, Sinha RP. Solar ultraviolet radiation-induced DNA damage in aquatic organisms: potential environmental impact. Mutat Research, 2005，571(1-2):221-233.
[28] Iyer RR, Pluciennik A, Burdett V, et al. DNA mismatch repair: functions and mechanisms. Chemical Reviews,2006,106(2):302-323.
[29] Lo HL, Nakajima S, Ma L, et al. Differential biologic effects of CPD and 6-4PP UV-induced DNA damage on the induction of apoptosis and cell-cycle arrest. BMC Cancer, 2005:5:135.
[30] Garcin G, Douki T, Stoebner PE, et al. Cell surface expression of melanocortin-1 receptor on HaCaT keratinocytes and alpha-melanocortin stimulation do not affect the formation and repair of UVB-induced DNA photoproducts. Photochemistry Photobiology science, 2007, 6(5):585-593.
[31] Kunz BA, Cahill DM, Mohr PG, et al. Plant responses to UV radiation and links to pathogen resistance. International Review of Cytology, 2006, 255:1-40.
[32] 王睿,郭周义,曾常春.紫外辐射引起DNA损伤的修复.中国组织工程研究与临床康复,2008,12(2):1-8.
[33] Kimura S, Sakaguchi K. DNA repair in plants, Chemical. Reviews, 2006, 106:753-766.
[34] Olive PL, Banath JP. The comet assay: a method to measure DNA damage in individual cells, Nature Protocols, 2006, 1:23-29.