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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (11): 30-33.

所属专题： 资源与环境

• 生物技术科学 • 上一篇    下一篇

梨种质资源遗传多样性研究中的RAPD技术引物筛选

马艳芝,张玉星   

  • 收稿日期:2009-03-06 修回日期:2009-03-11 出版日期:2009-06-05 发布日期:2009-06-05

Screening of Primers in Genetic Diversity Analysis of Pyrus Germplasm by RAPD

MA Yan-Zhi ,   

  • Received:2009-03-06 Revised:2009-03-11 Online:2009-06-05 Published:2009-06-05

PDF (PC)

674

摘要： 以梨属44个种和品种为试验材料，利用RAPD技术从50个随机引物中筛选出18个多态性引物，用于梨种质资源遗传多样性研究。结果表明：所选出的多态性引物每个引物扩增出的条带数在4—11之间，扩增出的DNA片段分子量大多在450bp—2000bp之间，共扩增出118个条带，其中样品间相同的条带数有11条，呈现多态性的条带107条，多态百分率达90.8%。

关键词: 聚丙烯酰胺, 聚丙烯酰胺, 沙化草场, 颗粒组成

Abstract: 18 primers selected from 50 ones were identified to be high polymorphic and were applied on RAPD amplification of 44 Pyrus plants, for genetic diversity analysis of Pyrus germplasm. The amounts of bands were 4-11 and the molecular weight mainly was 450bp-2000bp. A total of 118 DNA bands were amplified, including 11 same bands and 107 polymorphic bands.