欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 417-419.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

三种暖季型草坪草基因组DNA的提取方法

黄春琼 刘国道   

  • 收稿日期:2009-05-07 修回日期:2009-05-31 出版日期:2009-09-20 发布日期:2009-09-20

An improved method of extracting genomic DNA from Warm season Turfgrass

  • Received:2009-05-07 Revised:2009-05-31 Online:2009-09-20 Published:2009-09-20

PDF (PC)

688

摘要：

采用改进CTAB法分别提取狗牙根(Cynodon dactylon)、结缕草(Zoysia spp．)、假俭草(Eremorchloa ophiuroides) 3种暖季型草坪草基因组DNA。得到的DNA经核酸蛋白质分析仪测得A260/A280值处于1.74~1.98之间、A260/A230集中处于1.74~1.99，经琼脂糖凝胶电泳检测各样品具有一条明亮主带并无拖尾现象。表明改进CTAB法提取得到的DNA完整性好，纯度高，适用于分子生物学的研究。

Abstract:

Genome DNA was extracted by the inproved method from leaves of Cynodon dactylon(L.) Pers,Zoysia spp,Eremorchloa ophiuroides.The detection performed on a nucleic acids and protein analyzer showed that A260/A280 were situated between1.74~1.98,A260/A230 were ranged from 1.74 to 1.99.The extracted products had a bright band and without tailing bands which could be observed through agar gel electrophoresis. These results showed that genomic DNA obtained by optimum methodWhich had a good integrity and high purity,and could be used in each kind of molecular biology research.