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中国农学通报 ›› 2004, Vol. 20 ›› Issue (6): 36-36.

所属专题: 生物技术 园艺

• 目次 • 上一篇    下一篇

葡萄简化组织培养外植体系的建立

崔 刚,李文超,巩艳青,孙德宝,孙仲序   

  • 出版日期:2004-12-05 发布日期:2004-12-05

Establishment of Grape Explant in Simplified Tissue Culture

Cui Gang, Li Wenchao , Gong Yanqing,Sun Debao,Sun Zhongxu   

  • Online:2004-12-05 Published:2004-12-05

摘要: 为了在简化组培条件下获得葡萄高效外植体系,对抑菌剂的使用浓度、浸泡时间和培养浓度进行了大量比较试验。试验结果表明:抑菌剂浸泡浓度为4%,浸泡时间为6h,培养浓度为0.2%时,葡萄外植体的成功率能够达到95%以上,所获外植体分化正常。

Abstract: To obtain high efficient grape explant system in simplified tissue culture, a lot of compared experiments were made on Bactericides concentration ,soaked time and concentration in medium. Experimental results showed that soaked in 4%for 6h and inserted in 0.2% medium, the succeed percentage of grape explant could arrived 95% above, and the differentiation was normal.