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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (20): 95-99.

• 食品 营养 检测 安全 • 上一篇    下一篇

定量PCR快速检测动物食品中沙门菌污染

岳昌武1,吕玉红1,2,刘坤祥1,陈泽慧3,白国辉4   

  • 收稿日期:2009-07-27 修回日期:2009-08-04 出版日期:2009-10-20 发布日期:2009-10-20
  • 基金资助:

    贵州省卫生厅科学技术基金

Rapid detection of salmonella bacteria contaminated animal food with Real Time PCR

  • Received:2009-07-27 Revised:2009-08-04 Online:2009-10-20 Published:2009-10-20

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摘要：

摘 要： 沙门氏菌是动物性食品中的重要食源性致病菌，建立其快速检测方法对动物性食品的质量控制具有重要作用。本研究根据沙门氏菌16srDNA、 dT fermentation等基因或DNA功能区序列设计PCR引物, 通过比较3种不同的基因组DNA提取方法，提取待检样品总DNA, 进行多基因定量PCR检测。结果表明，该方法简单快速且灵敏度高，在动物食品中的检测灵敏度可达10 CFU/g，整个检测时间在10h 以内。说明本方法对检测动物中沙门氏菌具有特异性高且灵敏、快速等特点，适用于快速、准确地检测动物中沙门氏菌的需要。

关键词: 土地利用规划, 土地利用规划, 管理一体化, 思想

Abstract:

【Abstract】　Salmonella is an important foodborne pathogenic bacteria in animal food, establish a rapid detection methods plays an important role in animal food quality control. Three protocols were used to extract total DNA from one food sample from animal，and DNA products from the three protocols were all amplified by the primer of 16S rRNA gene and dT fermentation with Real Time PCR. Results show that this method is simple, rapid and high sensitivity, the detection sensitivity in animal food can reach up to 10 CFU/g, suggeste such method may be used fast testing the salmonella pollution in animal food.