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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (4): 89-92.

• 林学 园艺 园林 • 上一篇    下一篇

北美红杉的标准化离体繁殖技术研究

彭绿春1,苏艳1,王丽花1,王继华2,瞿素萍1   

  • 收稿日期:2009-09-21 修回日期:2009-11-01 出版日期:2010-02-20 发布日期:2010-02-20
  • 基金资助:

    云南省人才培引项目

Study on Standardized Technology of In Vitro Propagation of Sequoia sempervirens (Larnb.) Endl. With Softwood

  • Received:2009-09-21 Revised:2009-11-01 Online:2010-02-20 Published:2010-02-20

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摘要：

以两种类型北美红杉（观赏型和用材型）的嫩枝为外植体，通过植株再生与生根条件的研究，建立起标准化的离体繁殖技术体系。研究发现：北美红杉是一种较易增殖的木本植物，其根的诱导对无机盐浓度敏感；两种类型增殖的适宜培养基均是MS + 6-BA 0.1mg/L + NAA 0.1mg/L + KT0.1 mg/L，而生根的最佳培养基是1/4MS + NAA 0.4 mg/L，但观赏型北美红杉较用材型生根效果好。

关键词: 仁用杏, 仁用杏, 营养利用, 抗旱生理, 抗寒生理, 花器官

Abstract:

Take two type(watching type and wooding type) of softwood as explant, by studying the plant regeneration and rooting condition , to establish standardized in vitro propagation technology system for Sequoia sempervirens (Larnb.) Endl. The result showed that Sequoia sempervirens (Larnb.) Endl is a kind of woody plant multiplication easily and the inorganic salt concentration is the key factor for its rooting; their optimal multiplication medium is MS + 6-BA 0.1mg/L + NAA 0.1mg/L + KT0.1 mg/L, and the best rooting medium is 1/4MS + NAA 0.4 mg/L, the watching type rooting better than the wooding type.