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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 69-73.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

一种高效提取鹧鸪茶基因组DNA的方法

李娟玲1,2,刘国民1,2,贾媛2,兰云峰2   

  • 收稿日期:2009-10-15 修回日期:2009-11-17 出版日期:2010-04-20 发布日期:2010-04-20

An Effective Method for Genomic DNA Extraction in the Mallotus Oblongiolus(Miq.) Muell.-Arg.

Li Juanling1,2, Liu Guomin1,2, Jia Yuan2, Lan Yunfeng2   

  • Received:2009-10-15 Revised:2009-11-17 Online:2010-04-20 Published:2010-04-20

PDF (PC)

1035

摘要：

本研究在常规的CTAB法基础上，对其中某些步骤和所用药品进行改良,以寻求一种能简便快速地排除多酚类、蒽醌类和甾体类化合物等物质干扰的方法。应用该改良CTAB法所得鹧鸪茶总DNA鹧鸪茶总的DNA长度接近21kb，OD260/OD280在1.8左右。以此DNA为模板可获得条带清晰、重复性好的ISSR-PCR扩增结果。

Abstract:

Based on the routine CTAB method, some steps and reagents used to obtain DNA, were modified in this study to develop a simple and rapid method for removing the interference of some chemicals, such as polyphenols, anthraquinones, sterids and so on. The molecular weight of DNA, isolated by modified CTAB method, was nearly 21 kb, and the OD260/OD280 was about 1.8. Using the DNA isolated by modified CTAB method as the template in ISSR-PCR, the excellent amplification result, with distinct band and fine repeatability, could be obtained.