Abstract:

【OBJECTIVE】In order to investigate the root differentiation capability of Ardisia crenata callus, seek a new way of high frequency of root regeneration,【METHOD】different types callus of Ardisia crenata were used in root differentiation test and made into paraffin sections for cytology observation，for root differentiation capability compare of different types callus and the effects of different medium and light conditions on root differentiation.【RESULTS】The results both showed that yellow granular callus and yellowish-green nubbly callus can differentiate root easier than others.【CONCLUSION】In the medium of 1/2MS as the basic medium, of which the concentration of inorganic salts had been reduced, root differentiation rate was the highest. The weak light was beneficial to rooting by comparison.