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中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 167-172.

• 农学 农业基础科学 • 上一篇    下一篇

“贝达”和“赤霞珠”叶片中可溶性糖类对光合作用的调节作用

谢鹏1,赵方贵1,侯丽霞1,张艳萍1,刘新2   

  • 收稿日期:2009-11-02 修回日期:2009-11-19 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    农业部948项目

Regulation of Soluble Sugars on Photosynthesis in “Beta” and “Cabernet Sauvignon” Leaves

  • Received:2009-11-02 Revised:2009-11-19 Online:2010-02-05 Published:2010-02-05

摘要:

以两个葡萄品种“贝达”和“赤霞珠”为材料,研究了开花期过后一年生枝条功能叶片光合指标;以及葡萄糖、果糖、淀粉等碳水化合物含量与转化酶和淀粉酶活性的变化与联系。结果表明:开花期过后,“贝达”和“赤霞珠”功能叶片的净光合速率和Rubisco羧化效率呈下降趋势;果糖含量基本不变,葡萄糖、蔗糖及总可溶性糖含量波动上升;中性转化酶活性变化较小,α-淀粉酶和β-淀粉酶活性分别在前期和后期较高。“贝达”和“赤霞珠”功能叶片的Rubisco羧化效率与净光合速率呈极显著正相关;与葡萄糖/(果糖+蔗糖)呈显著负相关。在“贝达”和“赤霞珠”功能叶片中葡萄糖含量与β-淀粉酶活性分别表现出极显著和显著的正相关性。推断,开花期后“贝达”和“赤霞珠”功能叶片中葡萄糖的积累可能主要是通过β-淀粉酶代谢途径产生,并对其光合作用产生了反馈抑制的效应。

关键词: 节瓜, 节瓜, 镰刀菌酸抗性, 体细胞突变体

Abstract:

Photosynthesis parameters, contents of carbohydrate such as glucose, fructose, and starch, activities of invertase and amylase, along with their relationships were studied in the functional leaves of one-year-old twigs of two grape cultivars “Beta” and “Cabernet Sauvignon” after the flower stage. Results showed that the apparent photosynthesis rate and the Rubisco carboxylation efficiency decreased after the flower period. Meanwhile, the fructose content kept constant but the contents of glucose and sucrose increased with fluctuation patterns in the two cultivars’ functional leaves. In the same stage, the neutral invertase had a small variation but the α-amylase and the β-amylase had higher activities in the former stage and the latter stage, respectively, in both “Beta” and “Cabernet Sauvignon” functional leaves. After the flower period, the Rubisco carboxylation efficiency had a highly significant positive correlation with the apparent photosynthesis rate, and a significant negative correlation with the ratio of glucose/(fructose+sucrose) in “Beta” and “Cabernet Sauvignon” functional leaves. There were highly significant positive correlation and significant positive correlation between the glucose contents and the activities of β-amylase in “Beta” and “Cabernet Sauvignon” functional leaves, respectively. It can be deduced that: after the flower stage, the accumulation of glucose in “Beta” and “Cabernet Sauvignon” functional leaves is mainly generated from the metabolism pathway of β-amylase, which bring about the feedback inhibition to the photosynthesis of the two cultivars’ functional leaves.