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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 286-288.

所属专题： 资源与环境

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

梨种质资源遗传多样性研究中的AFLP技术引物筛选

鲁凤娟   

  • 收稿日期:2009-11-02 修回日期:2009-06-15 出版日期:2010-03-20 发布日期:2010-03-20
  • 基金资助:

    梨属种质资源分子遗传连锁图谱的构建与遗传多样性分析

Screening of Primers in Genetic Diversity Analysis of Pyrus Germplasm by AFLP

  • Received:2009-11-02 Revised:2009-06-15 Online:2010-03-20 Published:2010-03-20

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摘要：

本研究对带有3个选择性核苷酸的EcoRI和MseI引物各8个所组成的64个引物组合进行筛选，选出了多态性高的引物10对用于正式扩增。所选出的引物组合共扩增出371个位点，其中多态性位点306个，占总位点数的82.5%，每个引物组合扩增的位点数变化在23-55个之间，平均37.1个。所筛选出的10个引物组合为梨属植物AFLP扩增多态性引物，可广泛用于梨属植物的AFLP扩增技术。中国梨属植物资源丰富，通过对AFLP多态性引物的筛选，为该技术在梨属种质资源中的应用提供参考。

关键词: 当代社会, 当代社会

Abstract:

In this study, 10 primer combinations selected from 64 ones were identified to be high polymorphic. The amounts of bands were 23-55.A total of 371 DNA bands were amplified, including 306 polymorphic bands. The10 primer combinations were high polymorphic primers, which could be applied widely on Pyrus L.By AFLP technique. AFLP technique was applied on germplasm genetic diversity.It was abundant in Pyrus germplasm resources in China. It might be helpful for studying Pyrus germplasmic resources by screening of primers on AFLP.