欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 34-36.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

双温多重PCR鉴定猪早期胚胎性别的研究

李小平 逄大欣 袁婷 高飞 武蓉 黄永业 段新平 谢万华   

  • 收稿日期:2010-01-15 修回日期:2010-03-13 出版日期:2010-07-20 发布日期:2010-07-20

The study of Sex identification of pig with Two-temperature multiple PCR

  • Received:2010-01-15 Revised:2010-03-13 Online:2010-07-20 Published:2010-07-20

PDF (PC)

1110

摘要： 摘 要：通过PCR扩增哺乳动物雄性决定基因SRY来鉴定胚胎的性别在畜牧业生产上已经得到广泛应用，优化技术路线，缩短鉴定时间对后期实验的成功有非常重要的意义。本实验根据猪的SRY基因和GAPDH（内标）基因序列，分别设计并合成两对引物，通过双温PCR反应实现对猪早期胚胎性别的快速鉴定，结果表明：母猪只能扩增出 404bp 的GAPDH基因片段，而公猪能同时扩增出309 bp 的 SRY基因片段和 404 bp 的GAPDH基因片段。阳性率为100 %，鉴定时间较常规时间缩短2个小时。表明该检测方法快速、准确。本方法为在转基因克隆动物研究中快速鉴定胎儿成纤维细胞系的性别提供了可靠的技术保证。

关键词: 水稻（Oryza sativa L.）稻米品质, 水稻（Oryza sativa L.）稻米品质, 淀粉合成酶

Abstract: Abstract:Amplification of mammalian male-determining gene SRY with polymerase chain reaction(PCR) to identify the gender of embryos, has been widely used in animal husbandry, optimizing the technical route to shorten the time has a very important significance in the latter part of the experiment. In this study, we designed and synthesized two pairs of primers according to pig SRY gene and GAPDH gene (internal standard) to rapidly identify gender of the early pig embryor by Two-temperature PCR reaction, results showed that:the 404bp of the GAPDH gene fragment can be amplified with the sow gene, while the 309 bp of the SRY gene fragment and 404 bp of the GAPDH gene fragmen can be simultaneously amplified with the boar gene. The positive rate was 100%, identification time is shorten 2 hours than the normal methords. This indicates that this detection method is fast and accurate, it provides a reliable technical assurance for sex identification of fetal fibroblast cell lines in the study of transgenic animals.