Abstract: The experiment results showed that sulfur deficiency, especially fertilized less sulfur than 0.18g/kg, decreased soluble-protein content in flue-cured tobacco leaves. Meanwhile the amino-acids content was decreased on the conditions that sulfur fertilizer was deficient, and the addition of sulfur fertilizer was beyond 0.18 g/kg. The sulfur fertilizer of 37.5~112.5 kg/hm2 improved effectively the flue-cured tobacco. And the treatment with 75.0 kg/hm2 was recommended for flue-cured tobacco, yield and income increased by 11.73%, 10.67% than that of CK. If sulfur fertilizers increased to 225.0 kg/hm2, yield and income decreased 18.77%, 17.14% respectively compared with the treatment of 75.0 kg/hm2, the ratio of high- rate leaves also decreased by 9.9%.