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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (20): 15-17.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸭疫里默氏菌环介导等温扩增检测方法的建立

陈红梅 程龙飞,施少华,傅光华,万春和,黄 瑜   

  • 收稿日期:2010-05-19 修回日期:2010-06-21 出版日期:2010-10-20 发布日期:2010-10-20
  • 基金资助:

    鸭传染性荚膜炎与大肠杆菌病快速鉴别诊断技术的建立与应用

Rapid Detection of Riemerella anatipestifer infection by Loop-Mediated Isothermal Amplification Assay

  • Received:2010-05-19 Revised:2010-06-21 Online:2010-10-20 Published:2010-10-20

PDF (PC)

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摘要：

根据鸭疫里默氏菌(Riemerella anatipestifer，RA) ompA基因设计一套引物，同时优化其反应条件，建立鸭疫里默氏菌感染的环介导等温扩增 (LAMP）快速检测方法。结果表明，LAMP法的最佳反应条件为反应温度65℃，反应时间80 min。

关键词: 王草 柱花草 杂交水牛 育肥, 王草 柱花草 杂交水牛 育肥

Abstract:

According to the ompA gene of Riemerella anatipestifer (RA), a set of specific primers were designed to amplify the special DNA sequences by LAMP. Moreover, the reaction conditions of LAMP were optimized. The results showed that the optimal reaction temperature and reaction time were 65℃ and 80 min, respectively.