Abstract: In order to get the optimal medium to culture Cinnamomum caphora (L.) Prest. in it, semi-ligneous stem sections of a dominant tree in a mature forest were used as explants and orthogonal layout L9(34) with the compound of three elements (macroelements, BA and NAA) with three levels was adopted in this study. Four weeks later, either axillary sprouts or callus or both were produced by the stem sections cultured in all media. The results showed that the tested three factors have significantly affected the growth of sprouts and callus. Macroelements in MS medium and the highest concentration of BA were the optimal for the growth of sprouts, whereas the optimal conditions to develop callus were the highest concentration of NAA and the lowest concentration of BA. In addition, the sprouts flourished when the ratio of BA/NAA was equal to 40. However, the optimal value of BA/NAA to develop callus was 5 which was the lowest one among the ratios. Although the optimal medium to develop axillary sprouts was MS+BA2.0(mg./L)+NAA0.05(mg./L) and the optimal medium to produce callus was 1/2MS+BA0.5(mg./L)+NAA0.1(mg./L) in this study, the concentration of BA could be increased to develop more sprouts and we could get more callus by increasing concentration of NAA either lower concentration of BA for further study.