Abstract: The major structure protein E2 gene of Classical swine fever was amplified by polymerase chain reaction (PCR).After the amplified fragments were cloned into the eukaryotic expression vector PEGPF-C1,the recombinant plasmids PEGPF-E2 were obtained. The insert position, the size and the reading frame were right by PCR, restriction digestion and the sequence analysis. The positive plasmids was transducted by liposome into PK-15 cell which origin from pig kidney. PCR indicated that E2 gene was in positive cell clone which selection by G418.Fusion protein expression observated by inverted fluorescence microscope. Classical Swine Fever Virus Antigen Test Kit detection indicated that express fusion protein is E2 gene code protein.