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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (4): 139-143.

• 林学 园艺 园林 • 上一篇    下一篇

根癌农杆菌介导马齿苋遗传转化体系的研究

王红艳 王鸿磊 王洪芹 黄群策 秦广雍   

  • 收稿日期:2010-07-16 修回日期:2010-09-25 出版日期:2011-02-10 发布日期:2011-02-10

Genetic Transformation of Portulaca oleracea L. Callus Mediated by Agrobacterium tumefaciens

  • Received:2010-07-16 Revised:2010-09-25 Online:2011-02-10 Published:2011-02-10

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摘要：

利用马齿苋的叶片诱导出愈伤组织，再以愈伤组织为受体建立了根癌农杆菌介导的遗传转化体系。利用该转化体系得到抗性愈伤组织后，对其进行GUS组织化学染色和PCR扩增鉴定，证实为转化子，表明根癌农杆菌介导外源基因转化马齿苋的愈伤组织是完全可行的，为以后通过基因工程手段进行马齿苋的改良奠定了基础。

关键词: 镉 氮肥 苋菜 品质, 镉 氮肥 苋菜 品质

Abstract:

The callus was induced from the leaves of Portulaca oleracea L., and the callus was used for the receptor of genetic transformation mediated by Agrobacterium tumefaciens. The resistant callus was screened and confirmed to be transformants by GUS histochemical assays and PCR. It showed that genetic transformation of Portulaca oleracea L. callus mediated by Agrobacterium tumefaciens was entirely feasible.