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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 345-348.

所属专题： 土壤重金属污染

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

2株高效石油降解真菌对石油污染土壤的修复效果研究

高晶 李德生 秦晓 唐景春 张清敏   

  • 收稿日期:2010-08-20 修回日期:2010-10-20 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    天津市农业科技成果转化与推广项目（项目编号：0801160），环境污染过程与基准教育部重点实验室开放基金；国家高技术研究发展计划项目(863计划, No. 2007AA061201)；中国科学院知识创新工程项目（kzcx1-yw-06-03）。

Effect of Two Petroleum Degradation Fungi on Remediation of Petroleum-Contaminated Soil

  • Received:2010-08-20 Revised:2010-10-20 Online:2010-12-05 Published:2010-12-05

PDF (PC)

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摘要：

为了研究真菌对石油污染土壤的修复效果，将从石油污染盐碱土壤中分离得到的2株高效石油降解真菌菌种扩大培养，制成混合菌剂。通过盆栽试验，以石油烃降解率、土壤多酚氧化酶活性以及土壤的微生物多样性等为指标，研究了添加5%混合菌剂对石油污染土壤修复的作用。结果表明：受试土壤的总石油烃含量为2.1×104 mg/kg的情况下，修复第56天，添加菌剂处理的石油烃降解率达33.13%，是对照处理的1.69倍；多酚氧化酶活性比对照处理提高了41.94%；微生物多样性得到了增加。因此，本研究证实了所获得的2株真菌对石油污染盐碱土壤有较好的修复作用。

关键词: 国土整治, 国土整治, 规划思想, 一体化管理

Abstract:

In order to study remediation of fungi on petroleum-contaminated soil, two fungi which were isolated from the petroleum polluted salt alkaloid soil with the petroleum degeneration ability were mixed with humic acid to form the microbial inoculum. The degradation efficiency, polyphenol oxidase activity and microbial diversity of the soil were monitored in pot experiments periodically. The results showed that in the case of tested the soil total petroleum hydrocarbon content of 2.1×104 mg/kg, the total concentration of petroleum hydrocarbon decreased by 33.13% after 56 days of incubation, which was 1.69 times higher than that of CK; polyphenol oxidase activity increased 41.94% than that of CK, stimulating both the abundance and diversity of soil microbe. As a result, this paper had demonstrated the effectiveness of the two strains of fungi on petroleum-contaminated soil.