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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (3): 268-268.

所属专题： 园艺

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紫外线杀灭营养液中黄瓜枯萎病病菌试验

刘向辉,刘 芬,周立刚,宋卫堂   

  • 出版日期:2005-03-05 发布日期:2005-03-05

UV Disinfection of Fusarium oxysporum f.sp.cucumerinum in Drain Water from Closed System

Liu Xianghui, Liu Fen, Zhou Ligang, Song Weitang   

  • Online:2005-03-05 Published:2005-03-05

摘要： 设计、试制了一种可以用于无土栽培循环营养液消毒的紫外线消毒系统，并对其杀灭黄瓜枯萎病病原菌的性能和效果进行了实验室研究。先将黄瓜枯萎病病原(Fusarium oxysporum f.sp.cucumerinum)菌株接种于PDA培养基上，培养后将其加入到营养液中进行紫外线照射。通过对紫外线照射前后的病原浓度进行计数，发现紫外线对黄瓜枯萎病病原有较好的杀灭效果。在营养液紫外线透射率(T10)为96.65%、病原浓度为6.87×102conidia/ml、最大流量为36 L/min时，灭菌率为100%；在营养液紫外线透射率(T10)为95.42%、病原浓度为1.14×105conidia/ml、最大流量为36L/min时，灭菌率为100%；在T10降低为11.58%、病原浓度为1.44×104conidia/ml、最大流速为35L/min时，灭菌率也在50%以上。

Abstract: In this paper, an installation of UV disinfection was designed, and its efficacy against the Fusarium oxysporum f.sp.cucumerinum which is a kind of root-infecting pathogens, was tested in lab. The cultures of Fusarium oxysporum f.sp.cucumerinum were grown in potato dextrose and introduced artificially to a tank that contained enough water. By examining the number of spores grown in the plate of potato dextrose agar before and after being disinfected, it was found that Ultra-violet radiation is effective against Fusarium oxysporum f.sp. cucumerinum. The pathogens were totally killed when the water(T10=96.65%) flowed at 36L/min and the pathogen concentration was 6.87×102conidia/ml. Even if the concentration of pathogen was 1.14×105conidia/ml, the spores could not survive when the water(T10=95.42%) flowed at 36L/min. And when the water(T10=11.58%) flowed at 7.9 L/min and 35L/min, the disinfection rate was 81.31% and 50%, respectively.