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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 143-147.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

新疆加工番茄品种遗传多样性的SSR分析

张 超 薛 琳 田丽萍 魏亦农 罗 静   

  • 收稿日期:2010-10-21 修回日期:2010-12-10 出版日期:2011-03-20 发布日期:2011-03-20

Analysis on Genetic Diversity Tomato Varieties Using SSR Maker

  • Received:2010-10-21 Revised:2010-12-10 Online:2011-03-20 Published:2011-03-20

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摘要：

选用已筛选出带型稳定的44对SSR引物，对新疆主栽以及美国引进的加工番茄品种进行遗传多样性分析，结果表明：在24个品种间共扩增了283个等位基因，每对引物的等位基因数变化范围在2~17之间，平均为4.422个；有效等位基因为189.638，平均为4.310；每个SSR位点的多态性信息量变化范围为0.140~0.915，平均为0.662；基因型多样性变化范围为0.325~2.620，平均值为1.438；24个品种间的遗传相似系数变幅为0.715~0.924，平均值为0.820，且95%的供试品种其遗传相似系数在0.715~0.800之间，亲缘关系较近。以遗传相似系数为原始数据，按UPGMA方法将供试品种划分为4大类群，结合系谱分析结果表明，新疆的加工番茄品种遗传多样不够丰富，多数品种间的亲缘关系较近，欲进一步提高新疆加工番茄的产量及品质性状还需拓宽亲本选择范围，扩大遗传背景。

关键词: 超低温冷冻保存, 超低温冷冻保存

Abstract:

The genetic diversity of 24 tomato varieties, which were bred by Xinjiang province or introduced from America, was analyzed by 44 SSR primers. The results showed that a total of 44 SSR primers performed polymorphism and 283 allele loci were detected. The average number of alleles per SSR locus was 4.422 with a range from 2 to17. The effective alleles were 189.638 with average 4.310. The value of allelic polymorphism information content ranged from 0.140 to 0.915, on an average of 0.662 per SSR marker. The average number of genotypic diversity was 1.438 with a range from 0.235 to 2.620. The genetic similarity of paired varieties ranged from 0.715 to 0.924 with average 0.820, and the genetic similarity of 95% tested materials ranged from 0.715 to 0.800. The results of UPGMA indicated that the 24 tomato varieties could be clustered into 4 groups. Based on the pedigree analysis, it was concluded that the tomato varieties in Xinjiang province had closer genetic relationships with each other and the genetic diversity was not enough at present. It is necessary to explore the genetic resources and enlarge the genetic background in order to increase the yield in current rice breeding.