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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (16): 35-40.doi: 10.11924/j.issn.1000-6850.2013-3320

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

NaOH处理对紫椴种子休眠解除及生理生化特性的影响

郑金辉 林士杰   

  • 收稿日期:2013-12-19 修回日期:2014-02-09 出版日期:2014-06-05 发布日期:2014-06-05
  • 基金资助:
    吉林省科技发展计划资助项目 “紫椴种子休眠解除与萌发过程中物质转化的研究” (20100579)。

Effects of NaOH Treatment on Breaking Dormancy and Physiological and Chemistry Characteristic of Tilia amurensis Rupr. Seeds

  • Received:2013-12-19 Revised:2014-02-09 Online:2014-06-05 Published:2014-06-05

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摘要： 研究NaOH处理打破紫椴种子休眠的效果及NaOH处理后对紫椴种子萌发过程中贮藏物质的影响。采用不同质量分数NaOH处理紫椴种子，结合变温层积，定期统计发芽势、发芽率，并在层积过程中定期随机取样进行贮藏物质的测定。结果表明：30%NaOH处理1.5 h对紫椴种子休眠解除效果最好，发芽势平均为38%，发芽率平均为71.66%，20%NaOH处理1.5 h及10%NaOH处理6 h效果次之。与对照相比，NaOH处理后种子在整个层积过程中，淀粉、可溶性蛋白和可溶性糖含量显著下降，淀粉酶活性变化剧烈，呈“W”型。说明NaOH处理能够有效地解除紫椴种子的休眠，同时有效地改善了种子的透水透气性，在一定程度上加速了紫椴种子休眠解除过程中生理生化反应的进程。

关键词: 急性毒性, 急性毒性

Abstract: The Tilia amurensis Rupr. seed without pericarp was researched by NaOH in order to discuss the mechanism of breaking dormancy, and the changes of storage substance content in the integrative treated. Germination potential, germination rate and storage substance content were determined during seed germination of Tilia amurensis Rupr. by socking in NaOH. The results showed, all of them could reach the goal, but the best method was treating with 30% NaOH for 1.5 h. The average germination potential and germination rate were 38% and 71.66%, 20% NaOH for 1.5 h and 10% NaOH for 6 h were the secondplace. Compared with control, the starch, soluble protein and soluble sugar declined rapidly after germination, and the amylase changed with“W”after treated by NaOH. NaOH treatments were used to break seed coat barrier and change reactions of physiology and bio-chemistry in Tilia amurensis Rupr. seeds effectively.