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中国农学通报 ›› 2004, Vol. 20 ›› Issue (1): 29-29.

所属专题: 生物技术 水稻

• 目次 • 上一篇    下一篇

mRNA差异显示技术在分离水稻基因中的应用

程艳军,,郭士伟,高东迎,刘蔼民,陶 澜   

  • 出版日期:2004-02-05 发布日期:2004-02-05

The Application of mRNA Differential Display in Isolation of Genes in Rice(Oryza sativa L.)

Cheng Yanjun, ,Gao Dongying, Liu Aimin,Tao Lan   

  • Online:2004-02-05 Published:2004-02-05

摘要: mRNA差异显示技术是分子生物学中分离克隆基因的有效技术。笔者叙述了mRNA差异显示技术的基本原理、存在的问题以及技术改进, 并主要阐述了该技术在分离水稻基因中的应用情况。

Abstract: mRNA differential display reverse transcription polymerase chain reaction(DDRT-PCR)is an effective technology in isolation and cloning genes in molecular biology. The primary principle, superiorities, shortages, and technological improvements were reviewed, and this paper mainly focuses on the application of the technology in the isolation of rice (Oryza sativa L.) genes.