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中国农学通报

中国农学通报 ›› 2004, Vol. 20 ›› Issue (4): 23-23.

所属专题： 生物技术； 油料作物

• 目次 • 上一篇    下一篇

蓖麻毒蛋白的分离纯化和毒理作用研究

曾佑炜 宋光泉 彭永宏 徐杰 梁关生   

  • 出版日期:2004-08-05 发布日期:2004-08-05

Purification and toxicity characterization of Ricin from Ricinus communis beans

Zeng You-wei, Peng Yong-hong, Song Guang-quan　Xu jie, liang guan-sheng   

  • Online:2004-08-05 Published:2004-08-05

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摘要： 经（NH4）2SO4沉淀, 分子筛层析和亲和层析等处理，从去壳蓖麻籽中分离纯化得到蓖麻毒蛋白, 在SDS-聚丙烯酰胺凝胶电泳中呈32kDa和34kDa两条蛋白带, 证明该蓖麻毒蛋白已被纯化至均一。 用等电聚焦法测出该蓖麻毒蛋白的等电点为pI 6.1和pI6.7。 高效液相色谱测出该蓖麻毒蛋白在非变性条件下有五个峰, 表明蓖麻毒蛋白可能存在五个不同的多聚态。 动物实验表明该蓖麻毒蛋白对小白鼠有较强的毒性，但对斜纹夜蛾幼虫无毒杀作用。

Abstract: Ricin was purified from Ricinus communis beans to apparent homogeneity by ammonium sulfate precipitation, Sepharose-4B affinity chromatography, and followed by molecular sieve chromatography. The purified preparation showed two protein bands with relative molecular weights of 32 kDa and 34kDa when subjected to sodium dodecyl sulfate-polyacrylamide gel eloctrophoresis. The isoelectric point of the purified Ricin was pI6.1 and pI6.7 determined by isoelectric focusing (IEF). There were five peaks when Ricin was subjected to high performance liquid chromatography (HPLC) under non-denaturing conditions, indicating this Ricin consisted of five different multimers. Purified Ricin revealed high toxicity to mouse, but had no any toxicity to Prodenia litura Fabricius larvae.