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中国农学通报 ›› 2005, Vol. 21 ›› Issue (12): 314-314.

所属专题: 生物技术

• 目次 • 上一篇    下一篇

锥花福禄考的组织培养及快速繁殖

高丽霞,孔旭晖   

  • 出版日期:2005-12-05 发布日期:2005-12-05

In Vitro Propagation of Phlox paniculata.L.

Gao Lixia, Kong xuhui   

  • Online:2005-12-05 Published:2005-12-05

摘要: 建立了完整有效的锥花福禄考复壮、快繁体系。用0.1%HgCl2对其顶端嫩茎消毒9min效果最佳。接种在 and MS +BA1.0+NAA0.1 +30g糖固体培养基中分化率最高,而在增殖、扩繁阶段MS +BA0.5+ IBA 0.3 +30g糖效果最好,采用瓶外生根技术移栽成活率高,同时缩短了试管苗的繁殖周期。

Abstract: An efficiency system of propagation in vitro of Phlox paniculata.L.was developed. The optimum sterilization of young shoots was 9 minutes with 0.1% HgCl2. The best effect of explants was cultured on MS +BA1.0+NAA0.1 +30g sugar and the best effect of multiplication was cultured on MS +BA0.5+ IBA 0.3 +30g sugar. The survival rate of transplant was the hightest by rooting in nurery and the propagation periods were the shortest.