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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (11): 66-70.doi: 10.11924/j.issn.1000-6850.2010-0697

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

丹皮酚对LLO诱导大鼠肠黏膜微血管内皮细胞分泌NO、ET-1的影响

陈希 吴启 顾进华 许剑琴 穆祥   

  • 收稿日期:2010-03-10 修回日期:2011-12-02 出版日期:2012-04-15 发布日期:2012-04-15

Effects of Paeonol on the Secretion of NO and ET-1 in Rat Intestinal Mucosa Microvascular Endothelial Cells Induced by LLO

  • Received:2010-03-10 Revised:2011-12-02 Online:2012-04-15 Published:2012-04-15

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摘要：

为了研究李斯特菌溶血素(LLO)对大鼠肠黏膜微血管内皮细胞(RIMVECs)分泌一氧化氮（NO）、内皮素-1（ET-1）的影响，阐释作为治疗李斯特菌病方剂中单味中药丹皮的有效成分丹皮酚(Pae)调节LLO诱导细胞分泌紊乱的作用机理，试验将培养的RIMVECs分为LLO+Pae组、LLO组、Pae组、空白组，通过MTT比色法测定细胞生长情况；硝酸还原酶法测定细胞上清液细胞因子NO浓度以及酶联免疫法检测ET-1浓度；并用原位杂交方法对结果进行验证；结果显示LLO组与其他各组相比：细胞增殖显著性下降；12小时内，NO、ET-1分泌量高于正常水平，并导致NO／ET-1比值失衡（下降）；而LLO+Pae组NO/ET-1的比值显著上调；由此可见，Pae可以通过改善LLO引起细胞因子NO、ET-1失衡，有效调节细胞微环境，部分解释了中药丹皮治疗李斯特菌病的作用机理。

关键词: 小城镇建设, 小城镇建设, 回龙观文化居住区, 矛盾, 政府缺位

Abstract:

To investigate the treatment mechanism of the paeonol (Pae) on the listeriosis and the secretion of NO and ET-1 in rat intestinal mucosa microvascular endothelial cells (RIMVECs) induced by listeriolysin O (LLO). RIMVECs were in culture divided into LLO+Pae, LLO, Pae and blank control group. Cells were assessed proliferation by MTT assay. The Changes of NO and ET-1 level were measured by the method of nitratase, ELISA and hybridization in situ. Compared with other groups, proliferation of LLO was very significantly decreased. The production of NO and ET-1 were enhanced for 12h, and NO/ET-1 ratio was lower. Pae could regulate imbalance of NO/ET-1 induced by LLO and improve the local microcirculation of intestine.