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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 230-235.doi: 10.11924/j.issn.1000-6850.2010-3779

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

枯草芽孢杆菌B-332菌株发酵条件的研究

刘雪 叶婧 穆长青 朱昌雄   

  • 收稿日期:2010-12-27 修回日期:2012-02-20 出版日期:2012-04-05 发布日期:2012-04-05
  • 基金资助:

    国家水体污染控制与治理科技重大专项;“十一五”国家科技支撑计划项目

Research on the Fermentation Conditions of Bacillus Subtillus B-332

  • Received:2010-12-27 Revised:2012-02-20 Online:2012-04-05 Published:2012-04-05

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摘要：

为了探明生防菌株枯草芽孢杆菌B-332的发酵条件及培养基配方，采用单因素和正交试验设计，通过摇瓶培养对枯草芽孢杆菌B-332菌株的发酵培养基和培养条件进行优化。结果表明，最适培养接种时间为18 h，优化后的培养基配方为：豆饼粉0.60 g/L、蔗糖0.25 g/L、硫酸铵0.07 g/L、柠檬酸三钠0.03 g/L、磷酸氢二钾0.003 g/L、硫酸镁0.005 g/L、硫酸亚铁0.0005 g/L；发酵条件为：温度30℃、摇床转速180 r/min、摇瓶装量80 mL/500 mL。在该综合条件下，发酵后的芽孢数为1.43×1011 cfu/mL，比初始条件的芽孢总数4.03×109 cfu/mL提高了34.48倍，为该菌株的工厂化打下了基础。

关键词: 红叶, 红叶

Abstract:

In order to ascertain the cultivated conditions and the optimal medial components of Bacillus subtilis B-332, the cultivated conditions for Bacillus subtilis B-332 were optimized by single factor and orthogonal experimental designs. The results showed that the optimal seed aged 18 h, and the optimal fermentation time was 72 h, the optimal medial components were composed of 0.60 g/L soybean oil meal, 0.25 g/L saccharose, 0.07 g/L ammonium sulfate, 0.03 g/L trisodium citrate, 0.003 g/L dipotassium phosphate, 0.005 g/L magnesium sulfate, 0.0005 g/L ferrisulphas. Under these conditions, numuber of spores was 1.43×1011 cfu/mL, and increased 34.48 times compared to initialization, and it formed the base for the factory.