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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (29): 18-22.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪ESR、FSH-β和PRLR基因合并基因型对产仔数的影响

王立辛 苏玉虹   

  • 收稿日期:2011-04-25 修回日期:2011-05-13 出版日期:2011-11-15 发布日期:2011-11-15
  • 基金资助:

    CFL2b基因促进肌纤维分化的分子机理研究

Effects of Combined Genotype of ESR 、FSH-β and PRLR on Litter Size in Pigs

  • Received:2011-04-25 Revised:2011-05-13 Online:2011-11-15 Published:2011-11-15

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摘要：

摘 要: 本试验以辽宁省三个引进猪种：长白、大白和杜洛克为对象，采用PCR-RFLP方法检测ESR、FSH-β和PRLR基因的多态性，分析三个基因的单基因及合并基因型对产仔数的影响。结果表明，ESR 、FSH-β和PRLR基因都存在AA、AB和BB三种基因型，在各猪群内基因型分布差异不显著(P＞0.05)，各基因均处于遗传平衡状态。单基因分析表明，ESR 和FSH-β基因型产仔数的最小二乘均值具有按AA、AB 、BB递增的趋势，BB型为优良基因型，而PRLR基因的优良基因型为AA型。ESR 、FSH-β和PRLR基因的合并分析表明BBBBAA为最优合并基因型，合并后效应显著（P＜0.05)或极显著（P＜0.01)，且合并基因型的遗传效应高于单基因型效应。因此，可以利用合并基因型对猪的产仔数进行标记辅助选择。

关键词: 高吸水树脂, 高吸水树脂, 傅立叶变换红外谱, 熔融喷雾法, 形貌特征, 粒径分布

Abstract:

Abstract: This study was designed to analysis the effects of ESR、FSH-β and PRLR genes on litter size in three introduced swine (Landrace、Large Yorkshire、Duroc )of Liaoning province, single genotype effects as well as combined genotype effects were all studied, PCR-RFLP were applied to detect polymorphism of three genes. The results showed that there were there genotypes: AA、AB and BB for ESR、FSH-β and PRLR gene, the distributed difference of genotype was not significant(P＞0.05)，it was in genetic equilibrium for three genes. The results of single genotype effects showed that all LSM of the litter size had a gradually increasing tendency AA、AB and BB in turn, BB genotype of ESR and FSH-β gene were the superior one, but it was AA for PRLR gene. The results of combined genotype effects showed that BBBBAA was the dominant combination, there were significant differences （P＜0.05）or very significant differences（P＜0.01）in the combined genotypes effects, and the combined genotypes effects was higher than single genotypes effects. In conclusion, reproductive traits of swine could be improved by applying marker-assisted selection of combining genotypes.