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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (12): 216-222.doi: 10.11924/j.issn.1000-6850.2011-2561

• 植物保护 农药 • 上一篇    下一篇

氟磺胺草醚降解菌F-12的分离鉴定及降解特性,

吴秋彩 王晓萍   

  • 收稿日期:2011-09-05 修回日期:2011-11-23 出版日期:2012-04-25 发布日期:2012-04-25

Isolation Identification and Degradation Characteristics of Fomesafen Degradation Bacteria,

Xiao-Ping Wang   

  • Received:2011-09-05 Revised:2011-11-23 Online:2012-04-25 Published:2012-04-25

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摘要：

为了研究氟磺胺草醚污染土壤的生物修复机理，利用富集培养技术从长期施用氟磺胺草醚的土壤中分离得到1株能够以氟磺胺草醚为唯一碳源生长的细菌，命名为F-12。通过菌落形态、生理生化特性和16SrDNA基因序列分析，初步鉴定菌株F-12为克雷伯氏菌属（Klebsiella sp.）。并分析了氟磺胺草醚的初始浓度、接种量、温度和pH值对菌株F-12降解氟磺胺草醚效果的影响，确定了最佳降解条件。结果显示，该菌在氟磺胺草醚浓度为100 mg/L、接种量为15%、pH为6.0、温度35℃条件下，培养2 d后对氟磺胺草醚的降解效率达到80%以上。具有应用到氟磺胺草醚污染土壤生物修复的能力。

关键词: 内切葡聚糖酶活力, 内切葡聚糖酶活力

Abstract:

In order to study the mechanism of bioremediation in fomesafen-contaminated soil, a fomesafen degradable strain was obtained from fomesafen -contaminated soil. F-12，a bacterium which could use fomesafen as sole carbon source for growth, was isolated from the soil of long term applied with fomesafen by enrichment culture. Base on colony morphology, physiological and biochemical characteristics and 16SrDNA gene sequence analysis, the strain F-12 was identified preliminarily as Klebsiella sp.. The effect of initial concentration of fomesafen, inoculation amount, temperature and pH on degradation efficiencies was studied. The optimal degrading conditions were: initial concentration of fomesafen 100 mg/L, inoculation amount of 15%, pH 6.0, respectively, under the optimal conditions, the degrading efficiency could reach more than 80% after2 days at 35℃, holding the potential of being applied in the fomesafen bioremediation of contaminated soils.