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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (12): 88-92.doi: 10.11924/j.issn.1000-6850.2011-2965

• 农学 农业基础科学 • 上一篇    下一篇

氯嘧磺隆降解菌的分离鉴定及其降解特性

姜岩 刘艳 刘艳 王晓萍   

  • 收稿日期:2011-10-13 修回日期:2011-11-23 出版日期:2012-04-25 发布日期:2012-04-25

Isolation, Identification and Degradation Characteristics of Chlorimuron-ethyl Degradation Bacteria

  • Received:2011-10-13 Revised:2011-11-23 Online:2012-04-25 Published:2012-04-25

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摘要：

为了解决土壤中氯嘧磺隆残留对土壤的危害问题，从长期施用氯嘧磺隆的土壤中分离出1株对氯嘧磺隆有降解作用的菌株。通过采用生理生化、16S rDNA鉴定及高效液相色谱法，研究了其生理生化特性和降解特性。结果表明，该菌株为假单胞菌(Pseudomonas sp.)，最适生长温度30~35℃，pH 5~6，最佳接种量为5%。最终得出结论该菌在最适生长条件下，以100 mg/L的氯嘧磺隆为唯一氮源的培养基中培养5天，其降解率达到80%以上，与L-7混合后其降解率提高到90%以上。

关键词: 农村饮水, 农村饮水, 政府管理, 改革建议

Abstract:

In order to solve the soil chloride-ethyl residue on the soil of hazards, from the long-term application of chlorimuron-ethyl in soil isolated one pair of chlorimuron-ethyl with degradation of the strain. Through the use of physiological and biochemical, 16S rDNA identification and high-performance liquid chromatography was to study the physiological and biochemical characteristics, and degradation characteristics. The results showed that, the strain of Pseudomonas, the optimum growth temperature of 30-35℃, pH 5-6, the best inoculum size was 5%. Ultimately concluded that, the optimum growth conditions in bacteria could be 100 mg/L of ethyl chloride as the sole nitrogen source in the medium, and cultured for 5 days, the degradation rate of 80%, mixed with its L-7 degradation rate to 90%.