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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (14): 23-26.doi: 10.11924/j.issn.1000-6850.2011-3204

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

银杏叶提取物对肉仔鸡淋巴细胞增殖及细胞因子水平的影响

黄其春 杨小燕 林淑慧 何玉琴 李 焰   

  • 收稿日期:2011-11-02 修回日期:2011-11-21 出版日期:2012-05-15 发布日期:2012-05-15
  • 基金资助:

    福建省科技计划重点项目“银杏叶提取物对肉鸡免疫功能影响的研究”

Effects of Ginkgo Biloba Extract on Lymphocyte Proliferation and the Level of Cytokines in Broiler Chicks

  • Received:2011-11-02 Revised:2011-11-21 Online:2012-05-15 Published:2012-05-15

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摘要：

为了研究银杏叶提取物(EGB)对肉仔鸡淋巴细胞增殖及细胞因子水平的影响。将360只1日龄健康AA肉用母雏鸡随机分成5组，每组6个重复，其中一组为对照组，饲喂基础日粮；其余4组分别饲喂添加0.009%金霉素及0.15%、0.20%和0.25% EGB的日粮，试验期为42天。测定肉仔鸡外周血和脾脏淋巴细胞转化率以及血清中细胞因子IL-1、IL-2、IFN-γ、TNF-α的含量。结果显示，日粮中添加不同水平EGB可促进肉仔鸡外周血和脾脏淋巴细胞增殖转化，提高血清中细胞因子IL-1、IL-2、IFN-γ、TNF-α的水平，并且在日粮中添加EGB的作用效果比添加金霉素的作用效果好。表明日粮中添加EGB可增强肉仔鸡的免疫功能。

关键词: 黄体生成素, 黄体生成素, 促性腺激素释放激素, 脉冲

Abstract:

In order to investigate the effects of ginkgo biloba extract (EGB) on lymphocyte proliferation and the level of cytokines in broiler chicks. Three hundred and sixty one-day-old healthy Arbor Acres female broiler chicks were divided into five groups, each with six replicates. The first group was the control group and fed a basal diet, while the other four groups were fed the same basal diet supplemented with 0.009% aureomycin or 0.15%, 0.20%, 0.25% EGB. The experiment lasted 42 days. Lymphocyte proliferation of peripheral blood and spleen and the level of cytokine IL-1, IL-2, IFN-γ, TNF-α in serum were determined. The results showed that adding EGB to diets of broilers could improved lymphocyte proliferation of peripheral blood and spleen and increase the level of cytokine IL-1, IL-2, IFN-γ, TNF-α in serum. The effect of adding EGB to diets was better than that of adding aureomycin to diets. The study suggested that adding EGB to diets could enhance the immunity of broilers.