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中国农学通报 ›› 2012, Vol. 28 ›› Issue (11): 284-288.doi: 10.11924/j.issn.1000-6850.2011-3291

所属专题: 生物技术

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

餐厨垃圾堆肥过程中水解酶活性变化的研究

牛俊玲 郑宾国 梁丽珍   

  • 收稿日期:2011-11-08 修回日期:2011-11-28 出版日期:2012-04-15 发布日期:2012-04-15
  • 基金资助:

    河南省高校青年骨干教师资助计划项目;河南省重点科技攻关项目

Study on the Change of Hydrolytic Enzyme Activity During the Composting of Kitchen Wastes

  • Received:2011-11-08 Revised:2011-11-28 Online:2012-04-15 Published:2012-04-15

摘要:

为了研究餐厨垃圾在静态强制通风条件下堆腐过程的矿质化进度和强度。以小麦秸秆作为膨松剂,比较了不同C/N比(20,25,30)条件下餐厨垃圾堆肥过程中各个阶段纤维素酶、蔗糖酶、脲酶、蛋白酶4种水解酶活性的变化情况。结果表明,不同C/N比处理的温度变化趋势基本相同,40℃以上高温期持续达10天左右。25C/N和30C/N堆肥条件下的纤维素酶活整体比20C/N要高,酶活高峰期出现滞后于温度高峰期;不同C/N条件下,蔗糖酶活性变化趋势基本相似,酶活高峰期与温度高峰期同步;30C/N条件下的脲酶活性峰值出现早于20C/N和25C/N,最终脲酶活性下降到比堆肥初期还要低的程度;高温对蛋白酶活性有抑制作用,25C/N条件下蛋白酶活性峰值出现晚于其他2个处理。从试验结果可以看出,不同营养条件下同类酶活性的变化趋势表现出一定的差异,与堆肥过程中有机物质的转化密切相关,可以作为判断堆肥腐熟度的因素之一。

关键词: 选择性毒力, 选择性毒力

Abstract:

In order to study the mineralization process of organic substances, the composting for kitchen wasting mixed with wheat straw was carried out by static forced ventilation and under the conditions of different C/N ratio as 20, 25, 30, and the activities of Cellulase, Sucrase, Urease and protein enzyme were tested respectively at the different stages of composting. The results showed that the variation trends of temperature was similar for C/N ratio conditions, the temperatures above 40℃ lasted for about 10 days. The Cellulase activities under the conditions of 25C/N and 30C/N were higher than those of 20C/N, and the peaks of enzyme activity were lagged behind of the temperature peaks. Under different C/N conditions, the variation trends of Sucrase activities mainly was similar to each other, and the peaks of enzyme activities was synchronous to the temperature peaks. The peak of Urease activity under condition of 30C/N was appeared earlier than those of other two treatments, and eventually, the Urease activity declined in the last stage of composting. The higher temperature could inhibited the activity of protein enzyme, and its peak under condition of 25C/N was showed later than those of other two treatments. It could be seen from the results that the activities of the same enzymes were different under different nutrition conditions, and they were closely related to the change of organic substances, So they could be as one of the factors for evaluating the compost maturity.

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