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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (21): 189-192.doi: 10.11924/j.issn.1000-6850.2011-3415

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

产5-氨基乙酰丙酸的光合细菌的分离鉴定及hemA基因扩增

王悦   

  • 收稿日期:2011-11-16 修回日期:2012-02-06 出版日期:2012-07-25 发布日期:2012-07-25

Screening and Identification of A Strain of Photosynthetic Bacteria with High 5-aminolevulinic Acid Production and hemA Gene Amplification

  • Received:2011-11-16 Revised:2012-02-06 Online:2012-07-25 Published:2012-07-25

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摘要：

为了获得5-氨基乙酰丙酸(ALA)的高产菌株，从多种生境分离出光合细菌，通过分光光度计法检测ALA含量，通过形态学分析及16SrRNA分析进行菌种鉴定，PCR进行hemA基因的检测。获得1株产ALA的菌株RB，确定其为光合细菌的红假单胞菌属(Rhodopseudomonas)的沼泽红假单胞菌(Rhodopseudomonas palustris)。其ALA产量为4.34 mg/L，进一步对该菌株进行hemA基因扩增，成功获得扩增产物。本实验可以成功分离出产ALA的光合细菌，并成功获得hemA扩增产物，为构建基因工程菌打下基础。

关键词: 促进作用, 促进作用

Abstract:

The aim was to obtain the photosynthetic bacteria with 5-aminolevulinic acid (ALA) production. Photosynthetic bacteria was isolated from a variety of habitats, ALA content was detected by spectrophotometer, the strain was identification by morphological analysis and 16SrRNA analysis, the gene hemA was detection by PCR. A strain RB with 5-aminolevulinic acid (ALA) production was obtained. It was Rhodopseudomonas palustris of Rhodopseudomonas with 4.34 mg/L ALA production. Moreover, the hemA gene fragment was amplified from the strain. The results showed that the photosynthetic bacteria with 5-aminolevulinic acid (ALA) production were obtained successfully. And also successfully obtained the hemA amplification products, which provide the foundation for constructing gene engineering bacteria.