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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (17): 23-27.doi: 10.11924/j.issn.1000-6850.2011-3446

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

BMPR-IB、BMP15、GDF9基因作为戴云山羊多胎性能候选基因的研究

刘远 李文杨 林仕欣 张晓佩 高承芳 林碧芬 董晓宁   

  • 收稿日期:2011-11-18 修回日期:2011-12-20 出版日期:2012-06-15 发布日期:2012-06-15
  • 基金资助:

    福建省省属公益类科研院所基本科研专项项目;福建省农业科学院青年人才创新基金项目

Studies of BMPR-IB , BMP15 and GDF9 as Candidate Genes for Fecundity in Daiyun Goat

  • Received:2011-11-18 Revised:2011-12-20 Online:2012-06-15 Published:2012-06-15

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摘要：

为初步探索戴云山羊多胎性能的分子机制。以控制部分绵羊品种高繁殖力的BMPR-IB、BMP15和GDF9基因作为候选基因，采用PCR-RFLP方法分析戴云山羊BMPR-IB、BMP15、GDF9基因多态性与繁殖性状的关系。结果表明：多胎品种戴云山羊及南江黄羊在BMPR-IB基因的相应位置上并未发生与Booroola Merino羊相同的突变，同时也未检测到BMP15的FecXI、FecXH、FecXB基因、GDF9的FecGH基因，因此排除了这5个突变位点影响戴云山羊高繁殖力性状的可能性。因此，由于戴云山羊的BMPR-IB、BMP15、GDF9的全基因序列信息的缺乏，尚不能完全断定3个基因对戴云山羊高繁殖力性状没有影响。

关键词: 驱动力, 驱动力

Abstract:

To study the Molecular Mechanisms of fecundity trait in Daiyun Goat. BMPR-IB, BMP15 and GDF9 which control the fecundity of sheep were studied as candidate genes. PCR-RFLP was applied to analyze the relationship between the gene and genotype polymorphisms of BMPR-IB, BMP15 and GDF9 in Daiyun Goat and their prolific trait. The results showed that there was no same mutation in BMPR-IB gene of Daiyun Goat as that of Booroola Merino. The FecXI, FecXH , FecXB of BMP15 and FecGH of GDF9 were not found. Then it was ruled out the possibility that the fecundity of Daiyun Goat were affected by the mutations of BMPR-IB, BMP15 and GDF9 gene. As we didn’t knew the whole gene sequence of the three genes in Daiyun Goat, it’s hard to determine whether these genes affected the fecundity of Daiyun Goat.