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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (22): 130-137.doi: 10.11924/j.issn.1000-6850.2011-3562

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

白菜SAUR基因家族的生物信息学分析

赵敬会 张涛   

  • 收稿日期:2011-11-29 修回日期:2012-04-09 出版日期:2012-08-05 发布日期:2012-08-05
  • 基金资助:

    重庆市高等教育教学改革研究项目

Bioinformatics analysis of SAUR gene family in Brassica rapa

  • Received:2011-11-29 Revised:2012-04-09 Online:2012-08-05 Published:2012-08-05

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摘要：

旨在为今后开展白菜SAUR基因家族的功能奠定基础。本研究利用生物信息学的方法对白菜生长素早期应答基因SAUR(Small auxin-up RNA)家族的保守基序、氨基酸等电点、分子进化以及表达模式等基本性质进行了分析。结果表明，白菜143个SAUR蛋白质中，有111个蛋白质偏碱性，31个偏酸性，有一个成中性；从对该基因家族构建的进化树中可以看出它分化出2个大的亚家族，其中一个亚家族还处在不断分化中；此外，找到61个SAUR基因的EST表达证据，且基因表达部位比较丰富。通过研究分析表明本研究可以得出结论基因重复是白菜SAUR基因家族的一大特点，143个基因中含有51个同源对。

关键词: 气孔限制, 气孔限制

Abstract:

The aim was to lay the foundation for the function of auxin response genes in the SAUR gene family in the future. The conserved motif, isoelectric point of amino acids, molecular evolution, expression pattern and other basic properties of early auxin responsive gene SAUR family were analyzed by the bioinformatics method on Brassica rapa. The results showed that there were 111 alkaline proteins, 31 acidic proteins, and one neutral protein. The SAUR gene family divided into two major subfamilies from the phylogenetic tree, one of the subfamily was still in the continued differentiation. 61 SAUR genes were found EST evidence and the genes expression was rich in sites. Through research and analysis that gene duplication of the SAUR gene family was a major characteristic, 143 genes contained 51 homologous pairs.