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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 189-193.doi: 10.11924/j.issn.1000-6850.2012-0117

• 林学 园艺 园林 • 上一篇    下一篇

春兰名品杂交后代快繁与分化研究

孙芳 李承秀 张林 袁利利 于永畅 牛田 王长宪   

  • 收稿日期:2012-01-12 修回日期:2012-02-27 出版日期:2012-04-05 发布日期:2012-04-05
  • 基金资助:

    国兰新品种培育与产业化关键技术研究

Study on Rapid Propagation and Differentiation of Filial Generation of Cymbidium goeringii

  • Received:2012-01-12 Revised:2012-02-27 Online:2012-04-05 Published:2012-04-05

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摘要：

为了解决采用传统方法繁殖春兰速度慢的问题，对春兰名品‘集圆’与‘如意素’杂交获得的根状茎进行增殖与分化研究。设置不同的基本培养基、6-BA、NAA、IBA，探讨其对根状茎增殖与分化的影响。结果表明，1/2MS为春兰杂交根状茎的最适培养基；最佳增殖培养基为1/2MS+NAA 5.0 mg/L+6-BA 0.5 mg/L，1/2MS+NAA 0.5 mg/L +6-BA 2.0 mg/L对根状茎的分化效果最好。组培快繁与分化是加快新品种培育的重要方法与手段。

关键词: 内分泌干扰物, 内分泌干扰物

Abstract:

The aim was to study the rapid propagation and differentiation of rhizomes of Cymbidium goeringii ‘Jiyuan’ and ‘Ruyisu’. Four factors including basic medium, 6-BA, NAA and IBA were designed to study the effects on rapid propagation and differentiation of rhizomes. The most appropriate cultivating medium was 1/2MS. 1/2MS+NAA 5.0 mg/L+6-BA 0.5 mg/L was the best propagation medium and the rhizomes differentiates in high rate on 1/2MS+NAA 0.5 mg/L +6-BA 2.0 mg/L. Propagation and differentiation in vitro culture were the important means and methods for speeding up the cultivation of new varieties.

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