欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (3): 103-107.doi: 10.11924/j.issn.1000-6850.2012-0577

所属专题： 生物技术； 烟草种植与生产

• 农学 农业基础科学 • 上一篇    下一篇

NaCl胁迫对烟草种子萌发及幼苗生理特性的影响

李聪 郭梦阳 韩烈保   

  • 收稿日期:2012-02-25 修回日期:2012-04-12 出版日期:2013-01-25 发布日期:2013-01-25
  • 基金资助:
    引进国际先进林业科学技术948项目“转基因植物外源基因高效特异删除技术引进”（2011-4-50）；国家自然科学基金项目“麦冬ERF转录因子的克隆与功能研究”（31172255）。

Effects of NaCl Stress on the Seed Germination and Physiological Characteristics of Tobacco

  • Received:2012-02-25 Revised:2012-04-12 Online:2013-01-25 Published:2013-01-25

PDF (PC)

828

摘要： 研究不同浓度（质量分数）的NaCl (0、0.3%、0.6%、0.9%、1.2%、1.5%)对烟草种子萌发及幼苗生理特性的影响；测定了相对发芽势、相对发芽率、幼苗根长、以及叶绿素、细胞膜透性、丙二醛含量、脯氨酸含量；随着盐浓度增加，烟草相对发芽势、相对发芽率、根的长度、叶绿素含量呈现下降的趋势；细胞膜透性、脯氨酸含量则表现为持续升高趋势；丙二醛含量呈现先升后降的趋势；在盐浓度为1.2%时，烟草叶片叶绿素含量降幅最大，而此时丙二醛含量达到最大值，这对研究烟草抗盐机制提供了可靠依据。

关键词: 投影分析, 投影分析

Abstract: The aim was to investigate the effect of salt stress on the seed germination and physiological characteristics of tobacco. The relative germination potential, relative germination rate, root length, chlorophy ll content, cell membrane permeability, MDA content and proline content were measured. With the increase of NaCl concentration, the relative germination potential, relative germination rate, root length, chlorophy ll content kept decreasing; Cell membrane permeability and proline content kept increasing; the malondiadehyde content increased at first, and then decreased with the increase of NaCl concentration. When the concentration of NaCl was up to 1.2% , the tobacco leaf chlorophy ll content declined to the maximum extent and the malondiadehyde content reached maximum value which provided a reliable basis for the study of tobacco salt tolerance mechanism.