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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (20): 58-62.doi: 10.11924/j.issn.1000-6850.2012-0656

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

连翘酯苷对内毒素作用下RAW264.7细胞功能的影响

芦山 陈舒楠 官佳懿 沈红   

  • 收稿日期:2012-02-29 修回日期:2012-05-01 出版日期:2012-07-15 发布日期:2012-07-15
  • 基金资助:

    连翘提取物抗猪传染性胃肠炎病毒药效作用的研究

Effects of Forsythoside on Cell Functions of RAW 264.7 Stimulated by LPS

  • Received:2012-02-29 Revised:2012-05-01 Online:2012-07-15 Published:2012-07-15

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摘要：

为探讨连翘酯苷(FS)对内毒素(LPS)作用下RAW264.7细胞增殖、分泌NO和TNF-α、吞噬功能的影响。收集处于对数生长期细胞，用含10%胎牛血清的RPMI1640培养细胞，在培养液中分别加入脂多糖(LPS)以及不同浓度40、80、160 μg/mL的FS共培养。采用MTT法检测细胞增殖，Griess和ELISA法分别检测RAW 264.7细胞分泌NO和TNF-α量，染色法检测细胞吞噬能力。结果表明：低、中剂量FS可显著促进细胞增殖，而中和高剂量FS可缓解LPS对细胞的刺激作用；与LPS组比较，FS对LPS刺激的RAW 264.7细胞分泌NO影响不明显，但中、高剂量FS明显促进RAW 264.7细胞分泌；LPS与FS均能提高巨噬细胞吞噬鸡红细胞能力。FS对RAW264.7细胞增殖、分泌NO和TNF-α以及吞噬功能都有影响，结果提示这可能是连翘酯苷调节细胞免疫功能的机制之一。

关键词: 酶活力, 酶活力

Abstract:

To investigate the effects of Forsythoside (FS) on proliferation, NO secretion, TNF-α secretion and phagocytosis of RAW 264.7 cells stimulated by LPS. RAW 264.7 cells in the logarithmic phase were collected and cultured in RPMI1 640 containing 10% fetal blood serum in vitro and the stimulant Lipopolysaccharide (LPS). Different FS concentrations of 40, 80, 160 μg/mL were added individually in culture medium. Cell proliferation was detected by MTT, ELISA and Griess methods were detected TNF-α and NO levels, and cell phagocytosis were analyzed by staining. The results showed that low and medium dose of FS could significantly promoted RAW 264.7 cell proliferation, and the proliferation of cells stimulated by LPS was alleviated at medium and high dose of FS. Compared with LPS group, NO level secreted from LPS-stimulated RAW 264.7 cells treated with FS had not significant effect, but cells secretion of TNF-α was markedly promoted by high dose of FS. FS could enhanced macrophage phagocytosis to chicken red blood cells. FS might had an impact on proliferation, NO and TNF-a secretion, and phagocytic ability of RAW 264.7 cells, which might be one of mechanisms of FS to regulate cellular immune function.