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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (15): 170-174.doi: 10.11924/j.issn.1000-6850.2012-0696

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

中国东北部与河北省致病疫霉SSR基因型的组成与分析

吴艳清 蒋继志 郑旭 桂春爽 张紫肖 赵磊   

  • 收稿日期:2012-03-03 修回日期:2012-03-19 出版日期:2012-05-25 发布日期:2012-05-25
  • 基金资助:

    农业部行业专项—重要农作物疫病菌主要致病型及其分布研究

Composition and Distribution of SSR Genotypes of Phytophthora infestans in Hebei Provence and Northeast China

  • Received:2012-03-03 Revised:2012-03-19 Online:2012-05-25 Published:2012-05-25

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摘要：

为了解中国东北部致病疫霉基因型分布及变化规律。利用2对SSR引物Pi4B和Pi4G对2008—2010年期间采自于河北、黑龙江、辽宁和吉林的373株致病疫霉的SSR基因型进行分析，4个省份共鉴定出16种SSR基因型，分别为A-03、A-09、B-01、D-03、F-01、F-04、F-05、F-06、F-07、F-08、G-02、G-03、I-01、J-01、K-01和L-01，其中主导基因型为F-01，占所测菌株的67.83%，其次为F-06基因型(14.75%)；河北、黑龙江、辽宁和吉林4个省份SSR基因型的种类分别为11种、8种、4种和3种；16种基因型中，A-09、F-07、F-08、I-01、J-01、K-01和L-01等7种基因型在东北及河北地区属首次报道；2008—2010年期间，河北省和黑龙江省致病疫霉SSR基因型数量均有所增加，辽宁和吉林基因型种类变化不明显，但总体上东北地区和河北省致病疫霉优势基因型较单一，这为上述地区制定马铃薯晚疫病综合防治策略、尤其是品种的合理布局提供了一定的理论依据。

关键词: 显微镜检, 显微镜检

Abstract:

To understand the distribution and variation of Phytophthora infestans in northeast China, SSR genotypes of 373 P. infestans strains collected from Hebei, Heilongjiang, Liaoning and Jilin Provinces in 2008—2010 were analyzed using the SSR primers Pi4B and Pi4G. A total of 16 kinds of SSR genotypes were identified: A-03, A-09, B-01, D-03, F-01, F-04, F-05, F-06, F-07, F-08, G-02, G-03, I-01, J-01, K-01 and L-01. Among them, the dominant genotype was F-01, accounting for 67.83% in the measured strains, followed by the F-06 genotype (14.75%). The numbers of SSR genotypes in Hebei, Heilongjiang, Liaoning and Jilin were 11, 8, 4 and 3, respectively. 7 kinds of SSR genotypes, including A-09, F-07, F-08, I-01, J-01, K-01 and L-01 were the first reported in the Northeast and the Hebei Region in China. During 2008—2010, the numbers of P. infestans SSR genotype were increased significantly in Hebei and Heilongjiang Province, and changed slightly in Liaoning and Jilin. It was clear that dominant SSR genotype of P. infestans in northeast of China and Hebei Province was single and high ratio could provide the basis for making comprehensive protection strategy in potato production, especially for the rational distribution of potato varieties.

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