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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (23): 18-25.doi: 10.11924/j.issn.1000-6850.2012-0805

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

利用数字基因表达谱对小尾寒羊高繁性状相关基因的筛选研究

罗庆苗 秦晓玉 苗向阳   

  • 收稿日期:2012-03-09 修回日期:2012-04-20 出版日期:2012-08-15 发布日期:2012-08-15
  • 基金资助:

    转基因生物新品种培育科技重大专项;,国家高技术研究发展计划（863计划）项目;石羊河下游荒漠绿洲过渡带固沙植被退化与土壤演变的数量关系研究;中国农业科学院创新基金项目

The Study on Related Genes of High Fecundity Using Digital Gene Expression Tag Profilingin Small-Tail-Han-Sheep

  • Received:2012-03-09 Revised:2012-04-20 Online:2012-08-15 Published:2012-08-15

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摘要：

为了筛选小尾寒羊母羊FecBBFecBB型与FecB+FecB+型卵巢组织差异表达基因，探讨小尾寒羊品种内两者繁殖力差异的分子生物学机理。本实验利用数字基因表达谱技术分别检测了处于自然发情期的FecBBFecBB型与FecB+FecB+型小尾寒羊卵巢组织的基因表达情况，对两者卵巢组织基因表达谱进行了差异分析，并通过分子注释系统平台DAVID对差异表达基因进行了GO功能显著性富集类分析和Pathway显著性富集分析。结果表明：处于自然发情期FecBBFecBB型比FecB+FecB+型小尾寒羊卵巢组织差异表达基因为238条，主要涉及类固醇生物合成与代谢过程、甾醇生物合成与代谢过程、脂质运输与定位、泛素特异的蛋白酶活性、GTP酶调节活性和Ras蛋白信号转导调节等显著GO功能类和细胞内吞过程、Notch信号通路和嘧啶代谢等显著调控通路。结合已有文献报道，文章初步认为基因STAR、FLCN、TGFI1、INHA、INHBA与Notch信号通路可能是参与调控FecBBFecBB与FecB+FecB+小尾寒羊高低繁殖性状差异的重要基因和调控通路。

关键词: 年积累量, 年积累量

Abstract:

Digital Gene Expression Tag Profiling (DGE) technology was used to construct gene expression profile to screen differentially expressed genes of the ovary tissue between FecBBFecBB and FecB+FecB+ ewes of small-tail-han-sheep and investigate the molecular mechanism related to fecundity difference between them. GO (Gene Ontology) and the Pathway analysis were conducted on differentially expressed genes using a free web-based molecular annotation system DAVID. A total of 238 genes were identified to be differentially expressed genes. They were mainly involved in steroid biosynthetic and metabolic process, sterol biosynthetic and metabolic process, lipid transport and localization, ubiquitin-specific protease activity, GTPase regulator activity and regulation of Ras protein signal transduction etc. The enriched pathways mainly included endocytosis, Notch signaling pathway and pyrimidine metabolism. According to published papers, the genes including STAR, FLCN, TGFI1, INHA, INHBA and the Notch signaling pathway were considered as the most important genes and pathway involved in fecundity differences between FecBBFecBB and FecB+FecB+ ewes of small-tail-han-sheep.