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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (4): 114-117.doi: 10.11924/j.issn.1000-6850.2012-2586

• 林学 园艺 园林 • 上一篇    下一篇

应用正交设计法优选台湾金线莲快繁培养基

魏翠华 秦建彬 谢宇 陈沁   

  • 收稿日期:2012-07-23 修回日期:2012-08-12 出版日期:2013-02-05 发布日期:2013-02-05
  • 基金资助:
    福建省福州市科技计划项目 “珍稀药材金线莲快速繁殖与高优栽培模式研究” (2011-N-29)。

Optimization of the Rapid Propagation Medium for Anoectochilus formosanus by Orthogonal Design

  • Received:2012-07-23 Revised:2012-08-12 Online:2013-02-05 Published:2013-02-05

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摘要： 以台湾金线莲试管苗顶芽为外植体，研究基本培养基、6-BA、NAA、蔗糖4个因素对不定芽增殖培养与生根培养的影响，应用L9(34)正交试验设计优选出台湾金线莲快繁培养基。结果表明，4个因素对不定芽增殖与生根培养均有极显著影响。对不定芽增殖培养的影响为：6-BA>蔗糖>NAA>基本培养基。对生根培养的影响为：蔗糖>6-BA>基本培养基>NAA。最适宜的增殖培养基为：MS+6-BA 3.0 mg/L+ NAA 0.3 mg/L+蔗糖20 g/L。最适宜的生根培养基为：1/2MS+6-BA 1.0 mg/L+NAA 0.6 mg/L+蔗糖40 g/L。

关键词: 电子舌, 电子舌

Abstract: It was studied on four factors of basic medium, 6-BA, NAA and sucrose on adventitious buds proliferation culture and rooting culture to optimize the rapid propagation medium of Anoectochilus formosanus by orthogonal design L9(34), taking the apical buds of test-tube seeding as the explants. The results showed that: four factors had a very significant impact on the adventitious buds proliferation culture and rooting culture. The effect for proliferation culture: 6-BA> sucrose> NAA> the basic medium, the effect for rooting culture: sucrose> 6-BA> the basic medium> NAA. The optimizing medium for proliferation culture was MS+6-BA 3.0 mg/L+NAA 0.3 mg/L+ sucrose 20 g/L. The optimizing medium for rooting culture was 1/2MS+ 6-BA 1.0 mg/L+ NAA 0.6 mg/L+ sucrose 40 g/L.