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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (11): 16-19.doi: 10.11924/j.issn.1000-6850.2012-2953

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

SYBR Green Ⅰ实时PCR技术鉴定鸭源性成分的研究

杨丽霞 宋涛平 谢晓红 付冠艳 刘佩 彭新凯   

  • 收稿日期:2012-08-29 修回日期:2012-11-13 出版日期:2013-04-15 发布日期:2013-04-15
  • 基金资助:
    “十二五”国家科技支撑计划项目;湖南省科技重大专项

SYBR Green Ⅰ Real-time PCR for Detection of Duck-derived Components

  • Received:2012-08-29 Revised:2012-11-13 Online:2013-04-15 Published:2013-04-15

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摘要： 为建立灵敏、可靠的食品中鸭源性成分鉴定方法，确定了一对可特异地检测出牛肉中所掺杂鸭肉成分的引物对，建立了SYBR Green Ⅰ实时PCR技术检测鸭肉的方法。结果表明：利用SYBR Green Ⅰ实时PCR技术可检测到鸭肉DNA，通过熔解曲线能有效地区分特异性产物，检测灵敏度可达到1 pg DNA。同时利用该方法可检测出熟牛肉、鸭肉混合样品中的鸭肉成分。SYBR Green Ⅰ实时PCR方法能快速、特异、灵敏的检测鸭肉成分。

关键词: 单粒法, 单粒法

Abstract: To establish a method which was use to detect the duck-derived components, one pair of primer was chosen to detect the duck in beef, fluorescent SYBR GreenⅠ Real-time PCR was developed for detection of the duck. The results showed that, duck gene DNA fragment could be detected and specially analyzed by melting curves, the detection limit was 1 pg DNA. Duck could be detected by the method from cooked duck and beef mixture. SYBR Green Ⅰ Real-time PCR was the effective methods for detecting duck.

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