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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21): 206-209.doi: 10.11924/j.issn.1000-6850.2012-3082

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大叶南五味子多糖的分级与纯化

蒋红 李新国 王宏军   

  • 收稿日期:2012-09-10 修回日期:2012-10-12 出版日期:2013-07-25 发布日期:2013-07-25
  • 基金资助:
    KPS调控鸡免疫缺陷活性成分的结构解析及分子机制

Fractional precipitation and purification of Kadsura marmorata polysaccharides

  • Received:2012-09-10 Revised:2012-10-12 Online:2013-07-25 Published:2013-07-25

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摘要： 为了获得大叶南五味子多糖单体。用乙醇为沉淀剂对大叶南五味子多糖进行分级，Sephadex柱色谱梯度洗脱分离各级多糖，蒽酮-硫酸比色法测定糖含量、检测洗脱液吸光度值。乙醇分级沉淀结果显示从大叶南五味子多糖分到KPSⅠ~Ⅴ五个级分多糖，糖含量分别为76.51 %、78.36%、95.13%、92.41%、88.35%；Sephadex柱色谱对各分级多糖分离结果显示，KPSⅡ、Ⅲ、Ⅴ中所含多糖分子量基本一致，为纯多糖；KPSⅠ、Ⅳ中含有不同分子量多糖，其中KPSⅠ-1、KPSⅡ-1、KPSⅢ-1、KPSⅣ-6、KPSⅤ-1均为纯多糖。分级纯化的大叶南五味子多糖单体为其化学结构的分析奠定基础。

关键词: 超声波辅助有机溶剂法, 超声波辅助有机溶剂法

Abstract: In order to obtain the monomer of polysaccharide from kadsura marmorata polysaccharides (KPS). KPS were fractionally precipitated by the different concentration ethanol. The polysaccharide monomers were separated through Sephadex column chromatography and NaHCO3 solution gradient elution. The content of sugar and absorbance value of eluant component were detected by anthrone-vitriol colorimetric method. The results of fractionally precipitation showed that five fractional polysaccharides, which named KPSⅠ~Ⅴ, and the content of sugar was 76.51 %, 78.36%, 95.13%, 92.41%, 88.35%, respectively. The results of separation by Sephadex column chromatography showed that KPSⅡ, KPSⅢ and KPSⅤ contained the single molecular weight polysaccharide, respectively. However, KPSⅠ and KPSⅣ contained the different molecular weight polysaccharides, respectively. Those polysaccharide monomers, which included KPSⅠ-1, KPSⅡ-1, KPSⅢ-1, KPSⅣ-6 and KPSⅤ-1 separated by Sephadex colum, laid the foundation for the further research chemical structure analysis of KPS.

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