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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21): 61-65.doi: 10.11924/j.issn.1000-6850.2012-3373

• 农学 农业基础科学 • 上一篇    下一篇

苦荞SSR分子遗传图谱的构建及分析

杜晓磊 张宗文 吴斌 李艳琴 王安虎   

  • 收稿日期:2012-10-15 修回日期:2012-11-14 出版日期:2013-07-25 发布日期:2013-07-25
  • 基金资助:
    国际生物多样性中心合作项目;现代农业产业技术体系建设专项资金资助；十一五科技支撑

Construction of Genetic Linkage Map in tartary buckwheat (Fagopyrum tataricum) using SSR

  • Received:2012-10-15 Revised:2012-11-14 Online:2013-07-25 Published:2013-07-25

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摘要： 构建苦荞遗传连锁图谱，为今后有关苦荞基因组结构、重要农艺性状QTL定位、分子标记辅助育种和基因克隆等研究工作奠定基础。以栽培苦荞‘滇宁一号’和苦荞野生近缘种杂交产生的119份F4代分离材料为作图群体，利用SSR分子标记来构建苦荞的分子遗传连锁图谱。本研究构建的连锁图谱包含15个连锁群，由89个标记组成，其中偏分离的标记有22个，占24.7%，每条连锁群上的标记在2~16之间。连锁群长度在6.9~165.8 cM的范围，覆盖基因组860.2 cM，总平均长度9.7 cM。本研究构建了首张苦荞SSR遗传连锁图谱，为苦荞QTL定位、基因克隆、遗传选育等研究奠定了基础。

关键词: 九龙坡区, 九龙坡区

Abstract: Abstract: The present study was aimed to construct a molecular linkage map of tartary buckwheat, which would provide essential tools for investigating genomic structure and evolution, and facilitate QTL mapping, marker-assisted selection and map-based cloning of important gene. In this study, a population of F4 generation including 119 individual plants was derived from the cross between ‘Dianning 1’ and a tartary buckwheat wild species. SSR markers were used to construct the molecular genetic linkage map. The genetic linkage map consisted 15 linkage groups with 89 markers including 22 (24.7%) distorted markers in the whole map and 2-16 markers in each linkage group. The total length of the map was 860.2 cM. The length of linkage groups varied from 6.9 to 165.8 cM, with a mean marker interval distance of 9.7 cM between markers. The current study constructed a first tartary buckwheat genetic linkage map, which provided the basis for the research on gene location and molecular breeding of tartary buckwheat.