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中国农学通报 ›› 2013, Vol. 29 ›› Issue (8): 1-5.doi: 10.11924/j.issn.1000-6850.2012-3457

• 畜牧 动物医学 蚕 蜂 •    下一篇

病原微生物药敏检测方法的研究进展

刁菁 杨秀生 李天保 叶海斌 许拉 樊英 王淑娴   

  • 收稿日期:2012-10-23 修回日期:2012-11-16 出版日期:2013-03-15 发布日期:2013-03-15
  • 基金资助:
    山东省科技攻关计划项目“海水养殖病害简易快速诊疗技术的研究”(2010GHY10503);山东省优秀中青年科学家科研奖励基金计划项目“鱼类迟钝爱德华氏菌中草药多糖型微胶囊重组亚单位疫苗的研制”(BS2010NY021)。

Advances of Antimicrobial Susceptibility Testing Methods for Pathogenic Microorganism

  • Received:2012-10-23 Revised:2012-11-16 Online:2013-03-15 Published:2013-03-15

摘要: 抗生素敏感试验在当前病原微生物耐药性持续增长的形势下,已成为指导临床科学合理用药不可或缺的重要环节。因此,药敏检测方法的完善与发展是快速获取科学、准确微生物耐药谱的前提,也是实现有效临床治疗的重要保障。本研究介绍了当前应用于微生物药物敏感检测的常规方法,如纸片扩散法、稀释法、Etest法等,简要分析了它们之间的优缺特性,并对近年来一些新兴发展的快速药敏检测方法进行了总结以及展望了不同类型药敏检测方法的发展和应用趋势。

关键词: 农牧交错区, 农牧交错区

Abstract: With the increasing development of bacterial resistance to antimicrobial, Antimicrobial Susceptibility Testing (AST) had became an important and indispensable step for guiding reasonably therapy in clinic. So the perfection and development of AST methods were the premise of the acquirement of the scientific and accurate bacterial drug resistant spectrum, and also were the guarantee of the effective treatment in clinic. In order to provide reference materials for the development and perfection of AST methods according to the characteristics of application in different industries, the traditional AST methods were introduced, such as disk diffusion method, dilution method, Etest method and so on. Their characteristics, advantages and disadvantages were briefly analyzed, and the new and fast detection methods in recent years were concluded. In addition, the development and application trend of the AST method were also prospected.