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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (11): 29-33.doi: 10.11924/j.issn.1000-6850.2012-3526

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

miR-26a和miR-30d在牛不同组织中表达规律分析

孙广杰 戴立胜 袁宝 朱亚南 张阳阳 柳思源 任久生 徐靖博 李付娟 张嘉保   

  • 收稿日期:2012-10-31 修回日期:2012-11-12 出版日期:2013-04-15 发布日期:2013-04-15
  • 基金资助:
    现代农业产业技术体系建设专项资金资助

Analysis on the Expression Regulation of miR-26a and miR-30d in the Different Tissues of Cattle

  • Received:2012-10-31 Revised:2012-11-12 Online:2013-04-15 Published:2013-04-15

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摘要： 摘要：目的：检测miR-26a和miR-30d在牛不同组织中的相对表达水平，并应用生物信息学方法分析其靶基因，探讨两者在牛不同组织中可能发挥的作用。方法：以西门塔尔牛为研究对象，利用TRIzol一步法提取牛各组织总RNA后，根据miRBase提供的bta-miR-26a和bta-miR-30d成熟序列，设计特异性的反转录茎环引物以及荧光定量PCR引物，反转录获得cDNA后进行荧光定量PCR检测其在牛不同组织中的相对表达水平，并应用Targetscan、RNA22、miRDB等生物学软件预测其可能存在的靶基因。结论：miRNA-26a和miR-30d在牛心脏、肝脏等组织中均有不同程度的表达，miR-26a在心脏中表达量最高，其次在脾脏，在睾丸中的表达量最低。miR-30d在肾脏中表达量最高，其次在心脏，在睾丸中表达量最低。通过生物信息学方法分析miRNAs的靶基因，推测miR-26a和miR-30d可能参与调控机体的免疫、繁殖等活动。

关键词: 微观结构, 微观结构

Abstract: Abstract：[Objective] The current research aimed to study the relative expression levels of miR-26a and miR-30d in the different tissues, and applying bioinformatic methods to analyze the possible role in cattles.[Method] Total RNA was extractd by TRIzol in the different tissues of Simmental cattle. The relative expression levels of miR-26a and miR-30d were performed by RT-qPCR with specific stem-loop primers based on the mature sequence of bta-miR-26a and bta-miR-30d. The target gene of bta-miR-26a and bta-miR-30d were predicted by TargetScan、RNA22、miRDB.[Conclusion] miRNA-26a and miR-30d all have certain expression level in all kinds of detected tissues, of which miR-26a has the highest expression level in the heart, followed in the spleen, and lowest level in the testis; miR-30d has the highest expression level in the kidney, followed in the heart, and lowest level in the testis. Bioinformatics show that miR-26a and miR-30d may be involved in the regulation of the immune, reproductive activities and so on.